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为锂元素在水产免疫中的应用作了探讨。主要包括无机锂对光合细菌和罗非鱼的毒性,无机锂对光合细菌的生长和罗非鱼免疫因子的影响,含锂培养的光合细菌体外抑菌和体内对罗非鱼免疫因子的影响等。试验结果表明,锂对光合细菌最小抑菌浓度为50mg/kg,最小杀菌浓度为100mg/kg,在8mg/kg～12mg/kg间对光合细菌生长具有显著促进作用。锂对罗非鱼半致死浓度为0.5mg/kg,安全浓度为0.1mg/kg,无机锂可以提高罗非鱼血清(浆)中溶菌和抗菌活力、增加免疫细胞的核质比,但吞噬细胞活力和免疫细胞数量没有增加;含锂培养的光合细菌细胞破碎物体外具有显著抑制弧菌的能力,体内可以显著提高罗非鱼血清中溶菌活力。说明锂作为免疫激活剂,在水产上应用是可行的,同时可以用光合细菌作为锂的载体来消除无机锂对罗非鱼的毒性。
The application of lithium in aquatic immunity was discussed. Mainly including the toxicity of inorganic lithium to photosynthetic bacteria and tilapia, the effect of inorganic lithium on the growth of photosynthetic bacteria and the immune factors of tilapia, the bacteriostasis in vitro of photosynthetic bacteria containing lithium and the influence on the immune factors of tilapia in vivo . The results showed that the minimum inhibitory concentration of lithium on photosynthetic bacteria was 50 mg / kg, the minimum bactericidal concentration was 100 mg / kg, and the growth of photosynthetic bacteria was significantly promoted at 8 mg / kg ~ 12 mg / kg. Lithium has a lethal concentration of 0.5mg / kg for tilapia and a safe concentration of 0.1mg / kg. Inorganic lithium can increase the bacteriolytic and antibacterial activity of tilapia serum (plasma) and increase the ratio of nuclear to cytoplasm of immune cells, but phagocytic cells Vitality and number of immune cells did not increase; lithium-containing photosynthetic bacteria cell debris in vitro significantly inhibited the ability of Vibrio, in vivo can significantly increase the tilapia serum lysozyme activity. Lithium as an immune activator shows that the application of aquatic products is feasible, and can be used as a carrier of lithium photosynthetic bacteria to eliminate the toxicity of inorganic lithium on tilapia.