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目的:研究大豆异黄酮孕期和出生后暴露对子代雄性大鼠生殖系统发育相关指标以及ER-β受体表达的影响。方法:SD大鼠随机分为对照组(玉米油)、大豆异黄酮50、200、400 mg/kg体重和已烯雌酚(DES)0.1 mg/kg体重组,母鼠从妊娠0 d开始灌胃各组药品,子鼠21 d断奶后开始灌胃直至处死。子代雄性大鼠每组随机取6只分别在49 d、90 d处死,观察生殖系统发育相关指标并检测睾丸组织ER-β受体表达情况。结果:①对于不同年龄段子代雄鼠,大豆异黄酮各剂量组以及DES组每日平均摄食量均无显著性差异,但49～90日龄子鼠大豆异黄酮200 mg/kg体重、400 mg/kg体重组以及0.1 mg/kg体重DES组的食物利用率显著下降。②大豆异黄酮剂量为50 mg/kg体重以上时,不同年龄段的子鼠体重均显著下降(P<0.05)且90日龄子代雄鼠体重变化大于49日龄子代雄鼠。③随着大豆异黄酮剂量的增加,睾丸重量、睾丸精子头计数和每日精子生成量呈显著下降趋势且90日龄子鼠以上指标的变化比49日龄子代雄鼠变化更加明显。④随着大豆异黄酮剂量的增加,不同年龄段子代雄鼠各剂量组ER-β受体表达与对照组比较均呈上升趋势且90日龄子代雄鼠上升趋势大于49日龄子代雄鼠。结论:大豆异黄酮孕期和出生后暴露可干扰子鼠雄性生殖系统的发育,ER-β受体表达变化可能是其机制之一。
Objective: To study the effects of pregnancy and postnatal exposure to soy isoflavones on reproductive system development and ER-β receptor expression in offspring male rats. Methods: The SD rats were randomly divided into control group (corn oil), soybean isoflavones 50,200,400 mg / kg body weight and diethylstilbestrol (DES) 0.1 mg / kg body weight group, Each group of stomach drugs, offspring 21 d after weaning began to stomach until sacrificed. Six offspring male rats were sacrificed at 49 d and 90 d, respectively. The reproductive development related indexes were observed and the expression of ER-β receptor in testis was detected. Results: (1) There was no significant difference in average daily food intake of each dose group of descendants of soy isoflavones and DES in different generations of offspring, but 200 mg / kg of body weight and 400 mg of soy isoflavones of 49-90 days old mice / kg body weight and 0.1 mg / kg body weight DES significantly decreased the food utilization rate. (2) When the dose of soy isoflavone was above 50 mg / kg body weight, the body weight of the rats in different age groups were significantly decreased (P <0.05), and the body weight of the 90-day-old offspring rats was greater than that of the 49-day-old offspring. ③ With the increase of dosage of soybean isoflavone, testicle weight, testicle sperm head count and daily sperm production showed a significant downward trend, and the changes of above indexes in 90-day-old mice were more obvious than those in 49-day-old offspring. ④ With the increase of dose of soybean isoflavone, the expression of ER-βreceptor in each dose of male offspring in different age groups showed an upward trend compared with the control group, and the 90-day-old offspring male rats increased more than the 49-day-old offspring mouse. CONCLUSION: Exposure to soy isoflavones during pregnancy and postnatal period may interfere with the development of male reproductive system in rats. The change of ER-β receptor expression may be one of its mechanisms.