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目的探讨腹腔镜手术对子宫肌瘤患者体内蛋白质氧化、脂质过氧化的影响。方法将40例子宫肌瘤患者随机分为腹腔镜组和开腹组两组,每组20例。测定两组患者腹腔镜手术前、手术结束时(气腹解除后5min)及术后24h血浆晚期氧化蛋白产物(AOPP)、丙二醛、血浆谷胱甘肽过氧化物酶(GPx)活性和血浆总抗氧化能力(AOA)的水平,以及开腹手术术前、手术结束(缝合腹壁时)及术后24h的水平。结果(1)腹腔镜组手术结束时血浆AOPP、丙二醛水平高于术前[(69±13)μmol/Lvs(50±9)μmol/L,P<001;(252±055)μmol/Lvs(185±019)μmol/L,P<001],血浆GPx活性、AOA水平低于术前[(029±009)U/mlvs(062±027)U/ml,P<001;(090±024)mmol/Lvs(141±039)mmol/L,P<001];术后24h血浆AOPP、丙二醛、GPx与术前比较差异无统计学意义[(50±10)μmol/L,P>005;(205±068)μmol/L,P>005;(052±006)U/ml,P>005],但AOA水平仍低[(100±031)mmol/L,P<001]。(2)开腹组手术结束时血浆AOPP、丙二醛含量比术前略有升高[(53±100μmol/Lvs(52±70)μmol/L,P>005;(209±051)μmol/Lvs(183±064)μmol/L,P>005],GPx活性、AOA水平比术前略有下降[(051±017)U/mlvs(057±021)U/ml,P>005;(120±046)mmol/Lvs133±037,P>005];术后24h血浆AOPP、丙二醛与术前相比升高[
Objective To investigate the effect of laparoscopic surgery on protein oxidation and lipid peroxidation in patients with uterine fibroids. Methods 40 patients with uterine fibroids were randomly divided into laparoscopic group and open group two groups, 20 cases in each group. The levels of plasma advanced oxidation protein product (AOPP), malondialdehyde (MDA) and plasma glutathione peroxidase (GPx) activity before operation, at the end of operation (5 min after pneumoperitoneum) Plasma total antioxidant capacity (AOA) levels, as well as laparotomy before surgery, surgery at the end (suture the abdominal wall) and postoperative 24h levels. Results The levels of plasma AOPP and MDA in the laparoscopic group were significantly higher than those in the preoperative group [(69 ± 13) μmol / L vs (50 ± 9) μmol / L, P <001 and (252 ± 055) μmol / LPS (185 ± 019) μmol / L, P <001], plasma GPx activity and AOA level were lower than those before operation [(029 ± 009) U / ml vs 024) mmol / L vs 141 ± 039 mmol / L, P <0.001; There was no significant difference in plasma AOPP, MDA and GPx between the two groups after operation ([50 ± 10] μmol / L, P But the level of AOA was still low [(100 ± 031) mmol / L, P <001]. (2) The levels of AOPP and MDA in the laparotomy group were slightly higher than those before operation (53 ± 100μmol / L vs 52 ± 70μmol / L, P> 005; 209 ± 051μmol / The levels of GPx activity and AOA were slightly decreased compared with the preoperative values [(051 ± 017) U / ml vs (057 ± 021) U / ml, P> 005; ± 046) mmol / L vs133 ± 037, P> 005]. The levels of plasma AOPP and malondialdehyde increased at 24h after operation