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目的为研究无花果叶乙醇提取物和无花果叶水提取物体外抗新城疫病毒(NDV)作用。方法采用组织细胞培养技术,充分利用无花果叶提取物中的有效成分,在人上皮样癌(Hep-2)细胞株、地鼠肾(BHK21)细胞株和鸡胚成纤维细胞(CEF)上研究其抗新城疫病毒的作用。结果无花果叶提取物在体外对NDV具有明显的抑制和杀灭作用,药物的最小有效浓度(MIC)为0.5mg/ml,无花果叶乙醇提取物和无花果叶水提取物最大无毒浓度(TDO)分别为550mg/ml和50mg/ml。治疗指数(TI)分别为1100和100。结论无花果叶提取物对NDV具有抑制和杀灭的特异性。在医药食品领域的应用具有广阔的前景。
Objective To study the anti-Newcastle disease virus (NDV) effect of fig leaf ethanol extract and fig leaf water extract. Methods Tissue culture techniques were used to make full use of the active ingredients in fig leaf extract to study human epithelioid carcinoma (Hep-2) cell line, hamster kidney (BHK21) cell line, and chicken embryo fibroblast (CEF) cells. Its anti-Newcastle disease virus effect. Results The fig leaf extract had obvious inhibition and killing effect on NDV in vitro. The minimum effective concentration (MIC) of the drug was 0.5 mg/ml. The maximum non-toxic concentration (TDO) of fig leaf ethanol extract and fig leaf leaf water extract was significant. 550 mg/ml and 50 mg/ml, respectively. The therapeutic index (TI) was 1100 and 100, respectively. Conclusion The extract of fig leaves has specificity for the inhibition and killing of NDV. The application in the field of medicine and food has broad prospects.