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为筛选分离植物根组织特异表达基因,以枳根RNA为试验组,叶RNA为驱动组,构建了枳根消减文库。从该消减文库中共获得有效序列1 177个,其片段长度主要分布在200~500 bp;序列拼接后得到455个非重复序列,其中245个与已知基因匹配,具有结合功能、催化活性和转运活性等生物学功能,可参与植物的代谢、细胞生长、个体发育、应激反应等生物学过程。实时定量PCR检测结果显示这些基因中的主要乳液蛋白、早期结瘤素样蛋白和贝壳杉烯酸氧化酶等基因在根中高表达。
In order to screen and isolate the plant root tissue-specific gene, the root RNA was used as experimental group and the RNA as the driving group. A total of 1 177 effective sequences were obtained from this subtractive library, and their fragments were mainly distributed in length of 200 ~ 500 bp. 455 non-repetitive sequences were obtained after splicing, of which 245 were matched with known genes and had the function of binding, catalytic activity and transport Activity and other biological functions, can participate in plant metabolism, cell growth, individual development, stress response and other biological processes. The results of real-time quantitative PCR showed that the major milk proteins, early nodulation-like proteins and kaurene acid oxidase genes in these genes were highly expressed in roots.