论文部分内容阅读
目的探讨建立标准化猪急性心肌梗死模型方法。方法将27只中国实验用小型猪随机分成实验组及对照组,分别结扎左冠状动脉前降支上等流量点及中下1/3处。术中监测血流动力学指标;术前、术后1周、5周磁共振检查心脏结构及射血分数,5周后计算全心梗死体积。结果结扎点血流量与梗死体积及左心室EF值变异呈正相关。5周后dp/dt、左心室收缩末期及舒张末期内压(LVSBP及LVDBP)均显著降低(P<0.01)。两组心肌梗死体积(484.22±225.88)mm3比(2986.34±1937.13)mm3,P<0.01)、EF及dp/dtmax的变异差异均有统计学意义(P<0.05)。结论结扎LAD上等流量点可建立心肌梗死体积变异和心功能变异较小的急性心肌梗死动物模型。
Objective To explore a method to establish a standardized model of acute myocardial infarction in pigs. Methods Twenty-seven experimental miniature pigs in China were randomly divided into experimental group and control group, ligation of the anterior descending branch of the left coronary artery at the same flow rate and in the middle and lower third respectively. Intraoperative hemodynamic parameters were monitored. Cardiac structure and ejection fraction were measured before and 1 week and 5 weeks after operation. Total infarct volume was calculated after 5 weeks. Results There was a positive correlation between the blood flow of the ligation point and the infarction volume and the EF of the left ventricle. After 5 weeks, dp / dt, left ventricular end-systolic pressure and end-diastolic pressure (LVSBP and LVDBP) decreased significantly (P <0.01). The myocardial infarction volume (484.22 ± 225.88) mm3 (2986.34 ± 1937.13) mm3, P <0.01), and the differences of EF and dp / dtmax between the two groups were statistically significant (P <0.05). Conclusion LAD upper flow rate points can be established myocardial infarction volume changes and changes in cardiac function less acute myocardial infarction animal model.