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目的:建立玉屏风散的高效液相色谱指纹图谱。方法:采用HPLC法分析12批玉屏风散样品,确定指纹峰并用对照品随行进行定性和阴性对照随行进行峰归属鉴定,经过中药色谱指纹图谱相似度评价软件确定其对照指纹图谱,并对该方法进行方法学验证。结果:建立了由21个共有峰组成的玉屏风散高效液相色谱指纹图谱,指纹峰具有特征性,鉴定了其中11个主要的指纹峰,并对全部的共有峰进行了峰归属。结论:该文建立了玉屏风散高效液相色谱指纹图谱方法。结果可靠,重复性好,可全面有效地控制玉屏风散的质量。
Objective: To establish HPLC fingerprint of Yupingfeng San. Methods: Twelve batches of Yupingfeng powder samples were analyzed by HPLC, the fingerprint peaks were identified, and qualitative and negative controls were followed along with the reference substance to identify the peak assignments. The control fingerprints were identified by the similarity evaluation software of chromatographic fingerprints of Chinese traditional medicines. Methodological validation. Results: The HPLC fingerprint of Yupingfengsan was established with 21 common peaks. The fingerprint peaks were characteristic. Eleven major fingerprint peaks were identified, and the peaks of all the common peaks were assigned. Conclusion: This paper established a method of HPLC fingerprint of Yupingfengsan powder. The results are reliable, repeatable, and can effectively control the quality of Yupingfeng San.