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细胞骨架是真核细胞中的蛋白纤维网络体系,狭义的细胞骨架概念指细胞质骨架,包括微管、微丝、中间纤维等成分。微丝是肌动蛋白亚单位组成的螺旋状纤维(F-actin),在不同种类的细胞中,他们又与某些结合蛋白一起形成不同的亚细胞结构。考马斯亮蓝R250可以使各种细胞骨架蛋白染色,并非特异的显示微丝,但是由于有些细胞骨架纤维在该实验条件下不够稳定,而有些类型的纤维太细,在光学显微镜下无法分辨,因此,我们在光镜下看到的主要
Cytoskeleton is a network of protein fibers in eukaryotic cells. The narrow concept of cytoskeleton refers to the cytoplasmic framework, including microtubules, microfilaments, middle fibers and other components. Filaments are actin subunits composed of helical fibers (F-actin), in different types of cells, they together with certain binding proteins to form a different subcellular structure. Coomassie Brilliant Blue R250 stains various cytoskeletal proteins and does not specifically express microfilaments, but because some cytoskeletal fibers are not stable enough under these experimental conditions, some types of fibers are too thin to be resolved under a light microscope and therefore The main thing we see under the light microscope