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目的 观察凝固酶阴性葡萄球菌 (CNS)对甲氧西林等的耐药性和β-内酰胺酶的产生率 ,指导临床用药 .方法 对本院临床感染标本常规分离鉴定菌株 ,药敏试验应用 K- B纸片扩散法 ,按 NCCL S规定的标准进行 ,耐甲氧西林凝固酶阴性葡萄球菌 (MRCNS) MIC测定应用 Vitex微生物自动分析系统 ,β-内酰胺酶检测采用碘量试管法 .结果 CNS95株中 MRCNS的分离率为 39% ,β-内酰胺酶的阳性率为 6 3% ,MRCNS的产酶率为 95 % ,对甲氧西林敏感的葡萄球菌(MSCNS)的产酶率为 43% ;95株 CNS对万古霉素 10 0 %敏感 ,对氨苄青、青霉素、红霉素最低耐药率为 85 % ,对头孢哌酮、头孢唑林、舒普深最低敏感率为 83% .结论 MRCNS和MSCNS的产酶率与对甲氧西林的耐药性呈正相关 ,MRCNS与产 β-内酰胺酶葡萄球菌间的耐药率无显著差异 .目前万古霉素和舒普深是治疗 MRCNS和产酶 CNS首选抗生素
Objective To observe the drug resistance and the incidence of β-lactamase of coagulase-negative staphylococci (CNS) to methicillin and to guide the clinical use of drugs.Methods Strains were isolated and identified from clinical samples of infected patients, - B disk diffusion method, according to the standard NCCL S, methicillin-resistant coagulase-negative staphylococcus (MRCNS) MIC determination using Vitex automatic microbial analysis system, β-lactamase test using iodine test tube method Results CNS95 The isolation rate of MRCNS was 39%, that of β-lactamase was 63%, that of MRCNS was 95%, that of methicillin-sensitive staphylococcus aureus (MSCNS) was 43% 95 strains of CNS were sensitive to 10% vancomycin, the lowest rate of resistance to ampicillin, penicillin and erythromycin was 85%, and the lowest susceptibility to cefoperazone, cefazolin and shuxunshen was 83% .Conclusion MRCNS and MSCNS enzyme production rate and resistance to methicillin was a positive correlation between MRCNS and β-lactamase-producing Staphylococcus aureus rate was no significant difference between the current vancomycin and Shu Pu deep treatment of MRCNS and Producing enzymes CNS preferred antibiotics