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目的构建鼠疫耶尔森菌F1抗原蛋白的大肠杆菌分泌表达载体。方法 PCR法扩增鼠疫耶尔森菌的F1抗原基因,并插入大肠杆菌分泌表达载体p BAD/gⅢC中构建分泌表达载体。结果扩增出了长约500 bp的鼠疫耶尔森菌F1抗原基因并构建了该抗原的大肠杆菌分泌表达载体。结论本研究中我们用载体p BAD/gⅢC构建鼠疫耶尔森菌F1抗原的大肠杆菌分泌表达质粒,为后期制备针对鼠疫F1抗原的单克隆抗体或多克隆抗体,进一步建立便捷高效的鼠疫诊断方法奠定基础。
Objective To construct Escherichia coli secretion expression vector of Yersinia pestis F1 antigen protein. Methods The F1 antigen of Yersinia pestis was amplified by PCR and inserted into E. coli expression vector pBAD / gⅢC to construct the secretion expression vector. As a result, the Yersinia pestis F1 antigen gene of about 500 bp in length was amplified and the E. coli secretion expression vector of the antigen was constructed. Conclusions In this study, we constructed E. coli secreting expression plasmid of Yersinia pestis F1 antigen by using vector pBAD / gⅢC to further develop a convenient and efficient method for the diagnosis of plague for the preparation of monoclonal antibodies or polyclonal antibodies against plague F1 antigen Lay the foundation.