目的　鉴定重组卡介苗表达的MalE蛋白T细胞表位。方法　用抗原提呈试验、T细胞增殖试验、ELISPOT试验和表位作图测试重组MalE蛋白的T细胞表位。结果　重组BCG MalE功能性表达了MalE蛋白的 4种H 2 d 限制性T细胞表位。结论　在 4种表位中 ,p6 8～ 82是重组MalE蛋白的主要T细胞表位 ,而其余 3个为次要表位 Objective To identify the MalE protein T cell epitope expressed by recombinant BCG. Methods The T cell epitopes of recombinant MalE protein were tested by antigen presenting test, T cell proliferation assay, ELISPOT assay and epitope mapping. Results Recombinant BCG MalE functionally expressed the 4 H 2 d-restricted T-cell epitopes of the MalE protein. Conclusions Of the four epitopes, p6 8 to 82 are the major T cell epitopes of the recombinant MalE protein while the remaining three are minor epitopes