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目的探讨Nogo-A受体拮抗剂NEP1-40对Wnt信号通路和神经细胞增殖的调控作用。方法 40只大鼠被均分为HIBD(缺氧缺血性脑损伤)组和HIBD+NEP1-40组,采用PCR定量、Western blot分析、细胞增殖的免疫组化试验、8-异前列腺素评估等检测分析缺氧缺血性脑病新生大鼠的修复过程中Wnt信号通路中NgR的转录因子调控与神经细胞增殖。结果NEP1-40处理后,c Jun和c-Myc的表达在蛋白水平上调,基因表达水平上调,Ki-67增加,8-异前列腺素无显著变化。结论通过抑制NgR后发现,c-Jun和c-Myc是Wnt通路的主要转录因子,同时脑室下区神经细胞的增殖增加。
Objective To investigate the regulatory effect of Nogo-A receptor antagonist NEP1-40 on Wnt signaling pathway and neural cell proliferation. Methods Forty rats were divided into two groups: HIBD (hypoxic-ischemic brain damage) group and HIBD + NEP1-40 group. The expression of 8-isoprostaglandin was detected by PCR, Western blot analysis, immunohistochemistry, Et al. The transcriptional regulation of NgR and the proliferation of neural cells in Wnt signaling pathway during the repair of neonatal rats with hypoxic-ischemic encephalopathy were analyzed. Results After NEP1-40 treatment, the expression of c Jun and c-Myc were up-regulated in protein level, the gene expression level was up-regulated, Ki-67 was increased, but there was no significant change in 8-isoprostane. Conclusion By inhibiting NgR, c-Jun and c-Myc are the main transcription factors of Wnt pathway, meanwhile, the proliferation of neural cells in the subventricular zone is increased.