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目的:探讨葛根素对大鼠心肌缺血再灌注损伤的保护作用(Myocardial Ischemia-reperfusion Injury,MIRI)及抗氧化应激机制。方法:大鼠心肌缺血30min后再灌注180min造成大鼠心肌缺血再灌注损伤模型(灌注24 h,用于测定心肌梗死面积)。实验组和对照组分别给予葛根素和生理盐水。收集大鼠心脏,观察大鼠心肌缺血区的心肌细胞凋亡情况;收集血清测定其抗氧化应激的指标。结果:与模型组相比,葛根素剂量依赖性的减少了MIRI大鼠的心肌细胞凋亡、心梗面积和血清中丙二醛的含量,增加了血清中超氧化物歧化酶、谷胱甘肽过氧化物酶的活性和谷胱甘肽的含量。结论:葛根素对MIRI大鼠具有抗氧化应激的作用,它能够剂量依赖性的减少心肌细胞凋亡,最终减少心肌梗死面积。
Objective: To investigate the protective effects of puerarin on myocardial ischemia-reperfusion injury (MIRI) and the mechanism of anti-oxidative stress in rats. Methods: Myocardial ischemia-reperfusion injury was induced by myocardial ischemia for 30 min in rats and 180 min after reperfusion (24 h perfusion for determination of infarct size). Experimental group and control group were given puerarin and saline. The heart of rats was collected to observe the cardiomyocyte apoptosis in the ischemic myocardium of rats. Serum levels of anti-oxidative stress were measured. Results: Compared with the model group, puerarin reduced myocardial cell apoptosis, myocardial infarct size and the content of malondialdehyde in MIRI rats in a dose-dependent manner, and increased the contents of superoxide dismutase, glutathione Peroxidase activity and glutathione content. CONCLUSION: Puerarin has anti-oxidative stress effects on MIRI rats. It can reduce myocardial apoptosis in a dose-dependent manner and ultimately reduce myocardial infarct size.