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We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I(apoA-I) and ATP binding cassette transporter A1(ABCA1) on THP-1 cells.Single-molecule force spectroscopy shows that similar to normal apoA-I,the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells.The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5±4.9 pN.The minor direct apoA-I/ABCA1 binding strength is 56.7±4.1 pN.These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of apoA-I and ABCA1 at the single-molecule level.
We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein AI (apoA-I) and ATP binding cassette transporter A1 (ABCA1) on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA- the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5 ± 4.9 pN.The minor direct apoA-I / ABCA1 binding strength is 56.7 ± 4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of apoA-I and ABCA1 at the single-molecule level.