真皮及脂肪组织创伤后愈合差异性观察

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目的观察真皮和脂肪组织创伤愈合的差异性,探讨脂肪组织创伤在瘢痕形成中的作用。方法以杜洛克母猪为实验动物,用滚轴取皮刀在同一个体背部制作真皮及脂肪组织切割(n=6)或缺损(n=1)创面,采用自身配对观察比较创伤后1、2、4及12周的大体愈合情况。对于真皮及脂肪组织切割伤创面,于创伤后1、2、3周在创面中央取材,观察创伤处组织学改变,免疫组化法观察创面修复细胞表面分子Ⅰ型胶原α2、瘦素及α平滑肌肌动蛋白的表达。结果从创面中央看,瘢痕只出现在真皮组织切割伤和真皮组织缺损模型。真皮及脂肪组织切割伤同体配对比较结果显示,前者切缘处新生胶原沉积量要多于后者,且胶原类型存在差异;但两者创伤处修复细胞均以成纤维样细胞为主,细胞表面分子Ⅰ型胶原α2、瘦素及α平滑肌肌动蛋白呈阳性表达。结论真皮组织损伤或缺失是形成瘢痕的主要因素,而脂肪组织损伤或缺失与瘢痕的形成并无直接相关性。与真皮组织切割伤相比,脂肪组织切割伤的创伤处纤维化的程度较轻。 Objective To observe the difference of wound healing between dermal and adipose tissue and to explore the role of adipose tissue in the formation of scar. Methods Duroc sows were used as experimental animals. Dermal and adipose tissue (n = 6) or defect (n = 1) wounds were made on the back of the same individual using a roller and dermatome. , 4 and 12 weeks of general healing. For dermal and adipose tissue-incised wounds, the wounds were taken at the center of the wounds 1, 2, and 3 weeks after wounding to observe the histological changes of the wounds. The expression of collagen type α2, leptin and α-smooth muscle cells were observed by immunohistochemistry Actin expression. Results From the center of the wound, the scars only appeared in dermal cut and dermal defect models. The results of paired skin and fat tissue analysis showed that the amount of new collagen deposited at the margin of the former was higher than that of the latter, and the type of collagen was different. However, the repaired cells in the trauma were mainly fibroblast-like cells, The molecular type Ⅰ collagen α2, leptin and α smooth muscle actin were positive. Conclusions Dermal tissue injury or loss is the main factor in the formation of scars. However, the damage or absence of adipose tissue is not directly related to the formation of scars. Fibrosis is less severe at the wounds of adipose tissue cutting wounds than dermal tissue cutting wounds.
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