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目的:探讨八聚体结合蛋白4(Oct4)在食管鳞癌组织中的表达及其与食管癌患者预后的关系。方法:免疫组织化学SP法检测58例食管鳞癌及21例癌旁正常黏膜组织中Oct4蛋白的表达,分析其与各临床病理指标的关系;并结合Oct4表达情况及患者最短为期8年的随访资料,Kaplan-Meier法和Log-Rank检验分析Oct4与食管癌患者生存的关系;Cox回归模型评估Oct4及各病理指标与患者预后的关系。结果:食管鳞癌组织中Oct4阳性表达率为60.3%(35/58),显著高于正常组织中的19.0%(4/21),χ2=10.519,P=0.001;Oct 4的表达与患者年龄(χ2=0.023,P=0.879)、性别(χ2=0.043,P=0.836)、淋巴结转移(χ2=0.551,P=0.458)、外膜浸润(χ2=0.364,P=0.546)及UICC分期(χ2=1.038,P=0.308)均无相关性,但与组织学分级明显相关,χ2=6.061,P=0.014。死亡患者的Oct4表达程度也明显高于生存患者,χ2=7.958,P=0.005;且Oct4阳性表达患者的生存时间明显短于阴性表达的患者,χ2=7.703,P=0.006。Oct4表达水平与食管癌患者预后明显相关,RR=2.243,P=0.001。结论:Oct4在食管鳞癌组织中的表达水平与组织学分级及患者生存期相关,Oct4是影响食管癌患者预后的独立因素。
Objective: To investigate the expression of octamer binding protein 4 (Oct4) in esophageal squamous cell carcinoma and its relationship with the prognosis of patients with esophageal cancer. Methods: The expression of Oct4 protein in 58 cases of esophageal squamous cell carcinoma and 21 cases of adjacent normal mucosa was detected by immunohistochemical SP method. The relationship between the expression of Oct4 protein and the clinicopathological parameters was analyzed. Combined with the expression of Oct4 and the shortest 8-year follow-up Data, Kaplan-Meier method and Log-Rank test were used to analyze the relationship between Oct4 and the survival of patients with esophageal cancer. Cox regression model was used to evaluate the relationship between Oct4 and the prognosis of patients. Results: The positive rate of Oct4 expression in esophageal squamous cell carcinoma was 60.3% (35/58), significantly higher than that in normal tissues (19.0%, 4/21) (χ2 = 10.519, P = 0.001) (χ2 = 0.023, P = 0.879), lymph node metastasis (χ2 = 0.551, P = 0.458), adventitia (χ2 = 0.364, P = 0.546) and UICC stage (χ2 = = 1.038, P = 0.308), but it was significantly associated with histological grade, χ2 = 6.061, P = 0.014. The level of Oct4 in patients with death was significantly higher than that in patients with survival (χ2 = 7.958, P = 0.005). The survival time of patients with Oct4 expression was significantly shorter than that of patients with negative expression (χ2 = 7.703, P = 0.006). The expression level of Oct4 was significantly correlated with the prognosis of patients with esophageal cancer, RR = 2.243, P = 0.001. Conclusion: The expression of Oct4 in esophageal squamous cell carcinoma is correlated with histological grade and patient survival. Oct4 is an independent factor affecting the prognosis of esophageal cancer patients.