论文部分内容阅读
目的:观察PPARγ对人胰腺癌细胞中抑癌基因PTEN表达的影响。方法:体外培养人胰腺癌细胞株PANC-1,使用罗格列酮和GW9662细胞分别处理PANC-1细胞,RT-PCR检测PANC-1细胞中PTEN基因表达的变化;SABC法检测PTEN蛋白表达的变化,FCM法检测PTEN蛋白表达的百分率的变化。结果:PPARγ激动剂罗格列酮能够诱导抑癌基因PTEN的高表达,同时抑制剂GW9662能够抑制抑癌基因PTEN的表达,并呈一定的量效关系。结论:胰腺癌细胞中PPARγ的表达与抑癌基因PTEN的表达呈一定的平行关系,PPARγ可能通过一定信号转导通路调节抑癌基因PTEN的表达,从而发挥抗肿瘤的作用。
Objective: To observe the effect of PPARγ on the expression of tumor suppressor gene PTEN in human pancreatic cancer cells. METHODS: PANC-1 cells were cultured in vitro and PANC-1 cells were treated with rosiglitazone and GW9662 cells respectively. The expression of PTEN gene in PANC-1 cells was detected by RT-PCR. The expression of PTEN protein was detected by SABC method Changes, FCM method to detect the percentage of PTEN protein expression changes. Results: Rosiglitazone, a PPARγ agonist, could induce high expression of tumor suppressor gene PTEN. At the same time, GW9662 inhibited the expression of tumor suppressor gene PTEN and showed a dose-effect relationship. Conclusion: The expression of PPARγ in pancreatic cancer cells is in parallel with the expression of tumor suppressor gene PTEN. PPARγ may play an antitumor role by regulating the expression of tumor suppressor gene PTEN through certain signal transduction pathways.