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目的探讨足月窒息新生儿生后不同时段血清S100B蛋白的水平变化及与患儿听力损伤的相关性。方法足月窒息新生儿43例及足月正常新生儿27例,采用ELISA方法测定生后12、48 h及7 d血清S100B蛋白的水平;并于出生后5~7 d筛查瞬态诱发性耳声发射(TEOAE),35~42 d复查自动听性脑干反应(AABR),分别记录未通过的耳数,分析其与血清S100B蛋白之间的相关性。结果出生后12、48 h及7 d,重度窒息患儿血清S100B高于正常对照组[(0.138±0.039)μg/Lvs.(0.036±0.018)μg/L,(0.250±0.079)μg/L vs.(0.048±0.021)μg/L,(0.473±0.128)μg/Lvs.(0.049±0.277)μg/L](P<0.05);血清S100B与TEOAE的通过耳数呈负相关(P<0.05)。生后7 d血清S100B与AABR的通过耳数呈负相关(P<0.05)。结论血清S100B蛋白水平可以反映足月窒息新生儿早期的听力损伤的程度。
Objective To investigate the changes of serum S100B levels in neonates with full-term asphyxia after birth and its correlation with hearing loss in children. Methods Forty-three neonates with full-term asphyxia and 27 normal neonates were enrolled in this study. Serum levels of S100B protein at 12, 48 and 7 days after birth were measured by ELISA. Transient-induced Otoacoustic Emissions (TEOAE) and automatic auditory brainstem response (AABR) at 35-42 days were recorded. The number of failed ears was recorded and the correlation with serum S100B protein was analyzed. Results Serum S100B in children with severe asphyxia at 12,48 h and 7 d after birth was significantly higher than that in the control group [(0.138 ± 0.039) μg / L vs (0.036 ± 0.018) μg / L vs (0.250 ± 0.079) μg / L vs (0.048 ± 0.021) μg / L, (0.473 ± 0.128) μg / L vs (0.049 ± 0.277) μg / L], respectively (P0.05) .Serum S100B was negatively correlated with TEOAE . Serum S100B was negatively correlated with AABR passing ear number 7 days after birth (P <0.05). Conclusion Serum S100B protein level can reflect the degree of early hearing impairment of full-term asphyxia neonates.