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在硫酸介质,于温度100℃条件下,葡萄糖、果糖和木糖生成糠醛或糠醛衍生物,可与咔唑反应生成有色络合物。但络合物的吸收光谱重叠较严重,难以对单一组分进行测定。本文采用主成分回归法(PCR)解决光谱重叠的标准混合试样并建立校准模型。将此模型应用于葡萄糖、果糖和木糖模拟混合试样的同时测定。对模拟混合试样,回收率分别为99.2%,99.3%和100.7%。该方法应用于葡萄干样品中葡萄糖、果糖、木糖的测定,结果令人满意。
In sulfuric acid medium, under the conditions of temperature 100 ℃, glucose, fructose and xylose produce furfural or furfural derivatives, which can react with carbazole to form colored complex. However, the absorption spectra of the complexes overlap more severely and it is difficult to determine the single component. In this paper, principal component regression (PCR) to solve the spectral overlap of the standard mixed sample and establish a calibration model. This model was applied to the simultaneous determination of mixed samples of glucose, fructose and xylose. For the simulated mixed samples, the recoveries were 99.2%, 99.3% and 100.7%, respectively. The method was applied to the determination of glucose, fructose and xylose in raisin samples with satisfactory results.