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目的构建血管细胞黏附分子-1(VCAM-1)和绿色荧光蛋白(EGFP)双基因共表达重组腺病毒载体。方法采用 DNA 重组技术,将目的基因 VCAM-1克隆至含有报告基因 EGFP 的穿梭质粒;在 BJ5183细胞中与 pAdeasy-1质粒进行同源重组,产生重组腺病毒载体。结果 (1)VCAM-1基因与 pAdTrack-CMV 载体成功连接,经 Not
Objective To construct recombinant adenoviral vector co-expressing vascular endothelial cell adhesion molecule-1 (VCAM-1) and green fluorescent protein (EGFP) genes. Methods Recombinant adenoviral vector was constructed by cloning the target gene VCAM-1 into the shuttle plasmid containing EGFP reporter gene and homologous recombination with pAdeasy-1 plasmid in BJ5183 cells. Results (1) The VCAM-1 gene was successfully ligated with pAdTrack-CMV vector