【摘 要】
：
分离牛卵母细胞,分别用添加表皮生长因子(EGF)和发情牛血清(发情当天血清(OCS(0 d))和发情第7天血清(OCS(7 d)))的培养液进行成熟培养后去核,构建牛体细胞核移植胚并进行体外
【机 构】
：
西北农林科技大学,新疆石河子大学动物科技学院
【出 处】
：
西北农林科技大学学报（自然科学版）
【基金项目】
：
国家高技术研究发展计划(863计划);
论文部分内容阅读
分离牛卵母细胞,分别用添加表皮生长因子(EGF)和发情牛血清(发情当天血清(OCS(0 d))和发情第7天血清(OCS(7 d)))的培养液进行成熟培养后去核,构建牛体细胞核移植胚并进行体外培养,研究EGF和OCS对卵母细胞成熟及核移植胚体外发育的影响.结果表明EGF添加组和OCS(0 d)添加组卵细胞的成熟率及核移植胚的卵裂率和囊胚率分别为74.5%,84.6%,25.9%和83.9%,78.8%,31.3%.在牛卵母细胞成熟液中添加EGF、OCS(0 d)更有利于牛核移植胚胎的发育.
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