含抗人尿激酶单克隆抗体的腹水经ＰｒｏｔｅｉｎＡ－ＳｅｐｈａｒｏｓｅＣＬ４Ｂ柱纯化得到ＩｇＧ纯品，分子量为１５０ｋＤ（重链５５ｋＤ，轻链２０ｋＤ），与ＣＮＢｒ－Ｓｅｐｈａｒｏｓｅ４Ｂ偶联制成抗人尿激酶单克隆抗－体－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析介质。尿激酶粗品经单抗－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析柱纯化后，所得尿激酶制品比活为９×１０－４～１．７×１０－３ｍｏｌ／ｓ·ｍｇ－１，纯化倍数为１５～３０倍，酶活性回收率在５０％以上。 Ascites containing anti-human urokinase monoclonal antibody was purified by Protein A-Sepharose CL 4B column to obtain pure IgG with a molecular weight of 150 kD (heavy chain 55 kD, light chain 20 kD) and coupled with CNBr-Sepharose 4B to prepare anti-human urokinase monoclonal anti- Sepharose 4B affinity chromatography media. After purification of the crude urokinase by MAb-Sepharose 4B affinity chromatography, the specific activity of the urokinase product was 9 × 10-4 ~ 1.7 × 10-3mol / s · mg-1, the purification fold was 15 to 30 times , The recovery rate of enzyme activity is above 50%.