背景:臭牡丹属马鞭草科植物,现代药理学研究表明臭牡丹具有抗炎,抗肿瘤,提高机体非特异性免疫,兴奋子宫圆韧带肌电作用与激动α肾上腺受体有关,关于镇痛作用的实验研究较少。目的:通过热板法和扭体法观察臭牡丹的乙醇提取物对小鼠的镇痛作用。设计:以动物为观察对象,随机对照实验。单位:赣南医学院的药理学教研室。材料:实验于2004-03/06在赣南医学院药理教研室完成,选择昆明种小白鼠120只,体质量(20±2)g,由赣南医学院实验中心提供。干预:50只小白鼠用于扭体法实验,随机分为生理盐水组,氨基比林0.1g/kg组,吗啡0.01g/kg组,臭牡丹提取物20g/kg组,臭牡丹提取物40g/kg组,每组10只,分别经腹腔注射给药,40min后腹腔注射6mL/L乙酸溶液(10mL/kg),5min后开始观察小鼠扭体数和扭体抑制率,共观察10min。40只小白鼠用于热板法实验,随机分为生理盐水组,吗啡0.01g/kg组,臭牡丹提取物20g/kg组,臭牡丹提取物40g/kg组,每组10只,经腹腔注射给药后即刻置于热板金属表面,温度控制在(55±0.5)℃,分别记录给药后15,30,60min痛阈值。30只小白鼠用于对抗纳洛酮的热板法实验,随机分为纳洛酮0.04g/kg+吗啡0.01g/kg组,纳洛酮0.04g/kg+臭牡丹提取物40g/kg组,纳洛酮0.04g/kg+生理盐水组,经腹腔注射给药,记录给药后15,30,60,90min痛反应时间值。主要观察指标:①小白鼠扭体次数和扭体抑制率。②痛反应时间。③纳洛酮对抗后,痛反应时间。结果:120只小白鼠全部进入结果分析。①扭体次数和扭体抑制率:通过腹腔注射臭牡丹提取物20和40g/kg后小白鼠的扭体次数为2.4±2.5,0.6±1.7,它们对6mL/L乙酸溶液引起的疼痛反应的抑制率为93.3%,98.3%。②热板法实验给药后15,30,60min的痛阈值:给予臭牡丹提取物20g/kg后15,30,60min痛阈值为[(121.2±98.7)s,(191.2±78.6)s,(133.1±91.1)s];给予臭牡丹提取物40g/kg后15,30,60min痛阈值为[(233.9±70.4)s,(219.6±78.2)s,(218.3±92.6)s],均高于生理盐水组[(13.7±15.2)s,(9.7±12.5)s,(22.1±15.6)s](P<0.01～0.001)。③在纳洛酮对抗吗啡的实验中,吗啡0.01g/kg+纳洛酮0.04g/kg组给药后15,30,60min的痛域明显小于臭牡丹提取物40g/kg+纳洛酮0.04g/kg组[(1.7±5.2)s,(124.9±79.4)s,P<0.001;(6.4±8.6)s,(139.3±72.9)s,P<0.001;(21.8±34.0)s,(137.9±60.8)s,P<0.001]。结论:臭牡丹的乙醇提取物具有很强的镇痛作用,这种镇痛作用不是通过激动阿片受体而发挥镇痛效应的。 Background: The modern pharmacology study of the genus Perilla genus Verbena shows that the scented peony has anti-inflammatory and anti-tumor effects, enhances the body’s non-specific immunity, and stimulates the myoelectricity of the uterine round ligament to be associated with the activation of alpha adrenergic receptors. Less experimental research. OBJECTIVE: To observe the analgesic effect of the ethanol extract of stinky peony on mice by hot plate method and writhing method. Design: Animals are the subject of observation and randomized controlled trials. Unit: Department of Pharmacology, Wannan Medical College. MATERIALS: The experiment was performed at the Department of Pharmacology, Wannan Medical College from March to June 2004. 120 Kunming mice were selected and the body weight was (20 ± 2) g. The experiment was provided by the Experimental Center of Wannan Medical College. Intervention: 50 mice were used for the writhing test and randomly divided into normal saline group, aminopyrine 0.1g/kg group, morphine 0.01g/kg group, stinking peony extract 20g/kg group, stinking peony extract 40g In the /kg group, 10 rats in each group were intraperitoneally injected. After 40 minutes, 6 mL/L acetic acid solution (10 mL/kg) was intraperitoneally injected. After 5 minutes, the number of writhing and writhing inhibition of the mice were observed for 10 min. Forty mice were used for the hot plate experiment and were randomly divided into normal saline group, morphine 0.01g/kg group, stinking peony extract 20g/kg group, and stinking peony extract 40g/kg group, 10 in each group. Immediately after injection, they were placed on a hot plate metal surface and the temperature was controlled at (55 ± 0.5) °C. Pain thresholds at 15, 30, and 60 min after administration were recorded. Thirty mice were used for hot plate experiments against naloxone. They were randomly divided into naloxone 0.04g/kg+morphine 0.01g/kg group, naloxone 0.04g/kg+ stinking peony extract 40g/kg group, Lobenone 0.04g/kg+saline group was administered by intraperitoneal injection and the pain reaction time values ​​at 15, 30, 60, and 90 minutes after administration were recorded. MAIN OUTCOME MEASURES: 1 Writhing frequency and writhing inhibition rate of mice. 2 pain reaction time. After 3 naloxone confrontation, the pain reaction time. RESULTS: All 120 mice were involved in the analysis of the results. 1 Writhing frequency and writhing inhibition rate: The number of writhings of mice after intraperitoneal injection of S. peony extract 20 and 40 g/kg was 2.4±2.5, 0.6±1.7, and their pain responses to 6 mL/L acetic acid solution The inhibition rate was 93.3% and 98.3%. 2 Pain thresholds at 15, 30, and 60 min after the hot plate test administration: The pain thresholds at 15, 30, and 60 min after giving 20 g/kg of peony peony were [(121.2±98.7) s, (191.2±78.6) s, 133.1 ± 91.1) s]; The pain threshold at 15, 30, and 60 min after giving the extract of 40g/kg stinkberry was [(233.9 ± 70.4) s, (219.6 ± 78.2) s, (218.3 ± 92.6) s]. Saline group [(13.7 ± 15.2) s, (9.7 ± 12.5) s, (22.1 ± 15.6 s)] (P <0.01 to 0.001). 3 In the naloxone-antagonistic morphine experiment, the morphine 0.01g/kg+naloxone 0.04g/kg group had a significantly shorter pain zone at 15, 30, and 60 min after administration than the scented peony extract 40g/kg+naloxone 0.04g/ In the kg group [(1.7 ± 5.2) s, (124.9 ± 79.4) s, P <0.001; (6.4 ± 8.6) s, (139.3 ± 72.9) s, P <0.001; (21.8 ± 34.0) s, (137.9 ± 60.8) )s, P < 0.001]. Conclusion: The ethanol extract of stinking peony has strong analgesic effect. This kind of analgesic effect does not exert analgesic effect through the stimulation of opioid receptors.