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介绍了一种高通量水稻DNA微量快速抽提法。该方法特别适用于精细作图群体和大型突变体库的筛选鉴定,不仅通量大、速度快,而且可以确保足够的DNA浓度和纯度。DNA质量可以确保一般的PCR扩增,包括SSR检测以及应用于TILLING(targetinginducedlocal lesionin genome)分析。
A high-throughput DNA micro-extraction method was introduced. This method is particularly suitable for the screening and identification of fine mapping population and large mutant library. It not only has high throughput and high speed, but also ensures sufficient DNA concentration and purity. The quality of DNA ensures normal PCR amplification, including SSR detection as well as TILLING (targeting induced localized lesionin genome) analysis.