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目的 研究中药土贝母(BPF)水提物对体外培养的人肝癌细胞的抑癌活性。 方法 BPF经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得BPF水提物,人肝癌SMMC-7721细胞经BPF提取物处理不同时间后以MTT法测其对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。 结果 ①经200mg/L BPF提取物处理48h,人肝癌SMMC-7721细胞线粒体活性为对照组的31.3％,处理72h为21.4％;②以100mg/L BPF提取物处理7d中,人肝癌细胞数分别为0.57,0.316,0.29,0.285,0.57,1.287,1.2,对照FCS组分别为1.82,3.67,6.2,9.4,10.1,11,9,NON组分别为1.7,3.35,5.6,8.4,8.9,9.3,7.6,实验组人肝癌细胞数不仅低于对照组,而且低于处理前细胞数(P<0.01);③经BPF提取物处理24h后,即使更换为正常培养基,人肝癌细胞数在96h的恢复培养中基本无增加。 结论 中药BPF提取物能显著抑制体外培养的肝癌细胞增殖和降低线粒体代谢活性。
Objective To study the anti-cancer activity of the aqueous extract of the Chinese medicine Fritillaria cirrhosa (BPF) on human hepatoma cells cultured in vitro. Methods BPF was pulverized, leached, ultracentrifuged, concentrated under reduced pressure, and freeze-dried to obtain BPF aqueous extract. Human hepatocarcinoma SMMC-7721 cells were treated with BPF extract for different periods of time and their mitochondrial metabolic activity against human hepatoma cells was measured by MTT assay. The effect of cell number on cell proliferation was observed by cell counting. Results 1 After treatment with 200mg/L BPF extract for 48h, the mitochondrial activity of human hepatocellular carcinoma SMMC-7721 cells was 31.3% of the control group, and 21.4% after 72h treatment; 2 The number of human hepatoma cells was treated with 100mg/L BPF extract for 7 days. For the 0.57, 0.316, 0.29, 0.285, 0.57, 1.287, 1.2, FCS groups were 1.82, 3.67, 6.2, 9.4, 10.1, 11, 9, NON were 1.7, 3.35, 5.6, 8.4, 8.9, 9.3, 7.6, the number of human hepatoma cells in the experimental group was not only lower than the control group, but also lower than the number of cells before treatment (P<0.01); 3 After treatment with BPF extract for 24 hours, the number of human hepatoma cells was 96 hours even if it was replaced with normal medium. There is no increase in recovery culture. Conclusion The extract of traditional Chinese medicine BPF can significantly inhibit the proliferation of hepatocellular carcinoma cells in vitro and reduce the mitochondrial metabolic activity.