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在水稻的遗传转化中,迄今尚未见到将优良性状基因导入生产推广品种并获得转基因植株的报道。我们首次将B.t.杀虫基因导入到生产推广品种中花11号中,并建立了一套转导外源DNA的有效方法。应用剪掉内颖,同时去掉柱头保留完整外颖的方法可使种子结实率提高到50％。遗传转化植株经分子杂交实验室证实,B.t.杀虫基因已整合到水稻基因组中,得到了转杀虫基因水稻工程植株。用Jefferson的组织化学法测定转基因植株的β-glucuronidase的活性,与B.t.杀虫基因形成翻译融合的GUS基因得到了表达,这为B.t.杀虫基因在转基因水稻植株中的表达提供了证据。
In the genetic transformation of rice, so far no good trait gene introduced into the production of promotional varieties and reports of transgenic plants. For the first time, we introduced the B.t. insecticidal gene into Zhonghua 11, a production extension variety, and established a set of effective methods for transducing exogenous DNA. Application cut Ying, at the same time remove the stigma to retain the integrity of the method can make the seed set rate increased to 50%. Genetic transformation of plants confirmed by molecular hybridization laboratory, B.t. insecticidal genes have been integrated into the genome of rice, get insecticidal transgenic rice plant engineering. The Jefferson histochemical method was used to determine the β-glucuronidase activity of the transgenic plants. The expression of the GUS gene, which forms a translational fusion with the B.t. insecticidal gene, was obtained. This provided evidence for the expression of the B.t. insecticidal gene in transgenic rice plants.