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目的探讨环氧化酶-2(COX-2)选择性抑制剂塞来昔布(Celecoxib)对鼻咽癌细胞株HNE-1增殖与侵袭能力、血管内皮生长因子(VEGF)表达及放疗敏感性的影响。方法HNE-1细胞经不同浓度塞来昔布处理后,采用MTT法检测各组细胞的增殖水平,细胞侵袭试验检测细胞的侵袭转移能力,RT-PCR及ELISA分别检测细胞VEGF mRNA的转录水平及蛋白的表达水平,克隆形成试验检测细胞对放疗的敏感性。结果不同浓度的塞来昔布均可显著抑制HNE-1细胞的增殖与侵袭能力,并显著下调HNE-1细胞VEGF在mRNA及蛋白水平的表达,且均呈剂量依赖性,差异具有统计学意义。克隆形成试验结果表明,125μmol/L的塞来昔布与放疗联用对HNE-1细胞有明显的协同抗肿瘤效应。结论塞来昔布对HNE-1细胞的增殖与侵袭能力及VEGF的表达均有明显的抑制作用;经塞来昔布处理可增强HNE-1细胞对放疗的敏感性。
Objective To investigate the effects of Celecoxib, a selective inhibitor of cyclooxygenase-2 (COX-2), on the proliferation and invasion of nasopharyngeal carcinoma cell line HNE-1, the expression of vascular endothelial growth factor (VEGF) and radiosensitivity Impact. Methods HNE-1 cells were treated with different concentrations of celecoxib. The proliferation of HNE-1 cells was detected by MTT assay. The invasion and metastasis of cells were detected by cell invasion assay. The transcriptional level of VEGF mRNA was detected by RT-PCR and ELISA, respectively. Protein expression levels, clonogenic assay to detect cell sensitivity to radiotherapy. Results Different concentrations of celecoxib could significantly inhibit the proliferation and invasion of HNE-1 cells and down-regulate the expression of VEGF at mRNA and protein levels in HNE-1 cells in a dose-dependent manner . Clone formation test results show that 125μmol / L of celecoxib combined with radiotherapy on HNE-1 cells have a synergistic anti-tumor effect. Conclusion Celecoxib has a significant inhibitory effect on the proliferation and invasion of HNE-1 cells and the expression of VEGF. Celecoxib treatment can enhance the sensitivity of HNE-1 cells to radiotherapy.