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以乙型肝炎病毒(HBV)多聚酶 (P)逆转录酶 (RT)区及表面抗原 (HBsAg)主蛋白、e抗原 (HBeAg)氨基酸序列异质性来探讨HBV准种群在感染个体中的变异特点。应用多聚酶链反应 (PCR)方法自 8例慢性HBV患者血清中扩增靶基因 ,克隆入T载体 ,随机挑选 2 7株克隆测序 ,将获得基因的推断氨基酸序列进行比较后发现 :病毒结构/非结构蛋白氨基酸序列存在广泛的变异现象 ,替换突变表现出一定的个体特异性 ,其结果是导致HBV在特定的患者体内可能存在特征性的变异。本研究提示HBV在患者体内的蛋白序列多样性是乙型肝炎慢性化的一个重要原因。
In order to explore the variation of HBV quasispecies in infected individuals with the heterogeneity of amino acid sequence of hepatitis B virus (HBV) reverse transcriptase (RT), primary antigen of surface antigen (HBsAg) and e antigen (HBeAg) . The target genes were amplified by polymerase chain reaction (PCR) from the serum of 8 chronic HBV patients and cloned into T vector. Twenty-seven clones were randomly selected and sequenced. The deduced amino acid sequences of the obtained genes were compared and found to be: Amino acid sequences of structural proteins exist a wide range of variation. Substitution mutations show some individual specificity. As a result, there may be characteristic variations of HBV in specific patients. This study suggests that HBV protein diversity in patients is an important cause of chronic hepatitis B.