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糯稻89-1能通过腋芽休眠抗御低温越冬。以东乡普通野生稻(O.rufipogon)为对照品种,以死苗率为鉴定指标,对糯稻89-1和籼稻恢复系(明恢63,万恢86,蜀恢527,中413)构建的回交重组自交系(backcross inbred lines,BILs)群体(BC1F9)进行苗期耐寒性鉴定。结果表明,4℃低温处理48 h后然后在1/2 MS营养液中恢复生长7天,50个BILs群体中株系S176在3次苗期耐寒性鉴定中死苗率分别为5.2%,7.9%和9.4%,表明株系S176为强耐寒基因渗入系。利用人工气候箱进行低温处理,S176中可溶性蛋白、脯氨酸(Pro)、超氧化物歧化酶(SOD)含量呈上升趋势,并且都大于糯稻89-1;而丙二醛含量下降幅度且低于糯稻89-1;过氧化物酶(POD)含量在两者之间没有明显的差别。这些结果说明S176渗透物质比糯稻89-1积累快且幅度大,而细胞膜过氧化产物积累速度较慢,更有利于适应低温环境。
Glutinous rice 89-1 through axillary bud dormancy to resist cold overwintering. Using O. rufipogon as control, the rate of dead seedling was used as the identification index to evaluate the effects of glutinous rice 89-1 and indica restorer lines (Minghui 63, Wanhui 86, Shuhui 527, Zhong 413) Cold tolerance identification was performed at seedling stage in backcross inbred lines (BILs) populations (BC1F9). The results showed that after 40 ℃ low temperature treatment for 48 h and then in 1/2 MS nutrient solution for 7 days, the seedling rates of strain S176 in 50 BILs populations were 5.2%, 7.9% % And 9.4% respectively, indicating that the strain S176 is a strong cold tolerant gene introgression line. The content of soluble protein, proline (Pro) and superoxide dismutase (SOD) in S176 increased with the artificial climate chamber at low temperature, and both were higher than that of glutinous rice 89-1; however, the MDA content decreased and was low In waxy rice 89-1, there was no significant difference in peroxidase (POD) content between the two. These results indicate that S176 osmolytes accumulate faster and larger than that of glutinous rice 89-1, while the accumulation of peroxidation products in cell membrane is slower and more conducive to adapt to low temperature environment.