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目的:研究麻黄桂枝配伍对小鼠自主活动和脑内氨基酸水平的影响。方法:选择自主活动情况稳定的小鼠随机分为空白对照组、桂枝组(0.45 g/kg)、麻黄组(0.75 g/kg)和麻桂配伍组(1.5 g/kg),记录各组给药20,60,90,180,240 min后小鼠的水平活动次数,处死后迅速采集脑组织,采用高效液相色谱-荧光检测法进行小鼠大脑皮层内氨基酸含量测定。结果:与空白对照组比较,麻黄组(0.75 g/kg)小鼠在180 min时水平活动次数显著增加,麻黄与桂枝药对间存在交互作用,麻桂配伍组(1.5 g/kg)的水平活动次数较麻黄组有降低趋势。实验建立的色谱条件对脑组织匀浆液中17种氨基酸分离较好,日内和日间精密度的RSD分别<8.09%、8.70%,平均回收率均>81.68%。与空白对照组相比,在给药90 min后,麻黄组(0.75 g/kg)脑内抑制性氨基酸类神经递质(GABA、Ala)、缬氨酸(Val)、酪氨酸(Tyr)水平均明显降低,桂枝组(0.45 g/kg)、麻桂配伍组(1.5 g/kg)脑内GABA、Ala、Val、Tyr水品较麻黄组明显升高。结论:麻黄对小鼠中枢具有兴奋作用,桂枝的配伍能拮抗其中枢兴奋作用,其机制可能与抑制麻黄对脑内氨基酸水平的改变有关。
OBJECTIVE: To study the effect of compatibility of Ephedra cassia twig on autonomic activity and amino acid level in brain of mice. Methods: The mice with stable spontaneous activity were randomly divided into blank control group, Guizhi group (0.45 g / kg), Ephedra group (0.75 g / kg) and Maxillofaciens compatibility group (1.5 g / kg) After 20, 60, 90, 180 and 240 min administration, the number of horizontal movement of the mice was measured. The brain tissues were collected immediately after sacrifice and the content of amino acids in the cerebral cortex of the mice was determined by HPLC-fluorescence method. Results: Compared with the blank control group, the activity of Ephedra group (0.75 g / kg) significantly increased at 180 min, and the interaction between ephedra and Guizhi drug was significant The number of horizontal activities than that of Ephedra decreased. The established chromatographic conditions for the separation of 17 kinds of amino acids in brain tissue homogenate were better. The intra-day and inter-day RSD were <8.09% and 8.70%, respectively. The average recoveries were both> 81.68%. Compared with the blank control group, inhibitory amino acid neurotransmitters (GABA, Ala, Val, Tyr) in ephedra group (0.75 g / kg) The levels of GABA, Ala, Val and Tyr in Guizhi group (0.45 g / kg) and 1.5 g / kg group were significantly higher than those in Ephedra group. Conclusion: Ephedra has a stimulating effect on the central nervous system of mice. The compatibility of Guizhi can antagonize its central stimulant effect, and its mechanism may be related to the inhibition of ephedrine on the level of amino acid in the brain.