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稻米品质改良是水稻育种的重要方面,传统的常规育种方法由于缺乏对目标基因有效检测的手段,无法对稻米品质的基因型进行有效操作,因而品质改良进展缓慢.利用分子标记辅助选择与传统育种技术相结合,可以在对稻米外观品质进行鉴定的同时,对被选个体的基因型进行准确的鉴定.结合品质的测定,可以大大提高育种效率.但是,目前可用于稻米品质分子辅助育种的分子标记很少.稻米品质与淀粉组分密切相关,我们分析了16个典型水稻品种18个在淀粉合成中的重要基因的全基因序列,明确了各个基因在不同种质中的等位变异,设计了51个可以区分不同等位基因变异的分子标记,这可为稻米品质的分子育种提供可靠的依据.
The improvement of rice quality is an important aspect of rice breeding. The traditional methods of conventional breeding have not been able to effectively manipulate the genotypes of rice due to the lack of effective detection of the target genes, so the quality improvement is slow. The use of molecular marker-assisted selection and traditional breeding Technology can be combined to identify the quality of rice appearance at the same time, the selected individual’s genotypes for accurate identification.Combined with the quality determination, can greatly improve the breeding efficiency.However, the current molecular breeding can be used for rice molecular molecular breeding Few indications.Quality of rice and starch components are closely related, we analyzed the 16 typical rice varieties in the starch synthesis of 18 important genes in the gene sequence, each allele in different germplasm clear, the design of the Fifty-one molecular markers that could distinguish different alleles were provided, which could provide a reliable basis for molecular breeding of rice quality.