【摘 要】
：
Sillago sihama,commonly known as silver sillago,is considered as an economically important fish species in China.It is sensitive to hypoxia stress in the larval
【机 构】
：
Fisheries College,Guangdong Ocean University,Guangdong Research Center on Reproductive Control and B
【出 处】
：
中国海洋大学学报（自然科学英文版）
论文部分内容阅读
Sillago sihama,commonly known as silver sillago,is considered as an economically important fish species in China.It is sensitive to hypoxia stress in the larval stage,and the mechanism has not been understood thoroughly.In this study,we investigated the transcriptome change in heart tissues under hypoxia stress.The fish were divided into four groups,including 1 h of hypoxia (hy-poxialh,dissolved oxygen (DO)=1.5±0.1 mg L-1),4 h of hypoxia (hypoxia4h,DO =1.5±0.1 mgL-1),4 h of reoxygen (reoxygen4h,DO=8.0±0.2mgL-1) after 4h of hypoxia (DO=1.5mgL-1) and normoxia or control (DO=8.0±0.2mgL-1) groups.The results show-ed that a total of 3068 genes were identified as differentially expressed genes (DEGs) based on the criteria |log2(Fold change)| ＞ 1.0 and adjusted P-value ＜ 0.05.A total of 7761141 and 1151 DEGs were obtained from hypoxia l h,hypoxia4h and reoxygen4h groups,respectively.The enrichment pathway analysis showed that the DEGs were significantly enriched in ribosome biogenesis in eukar-yotes,retinol metabolism,DNA replication and the oxidative phosphorylation (OXPHOS) pathways.Thirteen DEGs from the RNA-seq results were validated by quantitative real-time polymerase chain reaction (qRT-PCR).These candidate genes are considered as important regulatory factors involved in the hypoxia stress response in S.sihama.
其他文献
The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of host response to HPND is of pivotal impor
为兼顾目标跟踪的准确度和目标跟踪时图像的处理速度,文章提出一种基于运动粒子的粒子群目标跟踪算法。该算法首先对目标跟踪区域提取HSV特征得到目标特征向量;然后以高斯分布的形式撒下n个粒子构成粒子群,通过梯度收敛算法可以快速准确地搜索到最佳的目标位置,并以此位置作为跟踪点,进行下一帧跟踪。实验结果表明,在精确度方面文章提出的算法是CSK算法的1.52倍、MS算法的1.57倍,在速度方面文章提出的算法是
文章介绍了新型强制风冷型鼓式制动的结构和降温机理。针对新型强制风冷型鼓式制动和原车制动毂,进行连续下长坡运行的制动毂温度升降对比试验,与原车制动毂相比,新型强制风冷式制动毂平均最高温度降低17℃,因未改变原车制动毂的结构,强度和刚度不会受到影响。即通过温度传感器感知制动毂温度,当超过150℃时,风机工作强制风冷,提高散热效果,制动效能明显改善。
The X-ray fluorescence(XRF)core scanning method is widely applied in studies of sedimentary paleoenvironments due to its convenient pretreatment,nondestructive characteristics,fast execution,continuous scanning,and high resolution.XRF core scanning for se
The dried shellfish products with rich nutrients and low-calorie content are favorite food in China,especially in coastal areas.However,the species of dried shellfish products in the market are usually unknown,as the taxonomic features were removed during
STAT3 plays a particularly important role in several cancer-related signal transduction pathways.Silencing STAT3 via RNA interference or small molecule inhibito
The mitochondrial genomes (mitogenomes) are purportedly under selection for smaller size to improve their replica-tion and translation efficiency.However,the mi
压力传感器在各大工厂的生产线上大量应用,由于工作环境高温,存在粉尘、水汽等情况,会导致压力传感器寿命缩短,设备出现故障.针对此问题文章以压力传感器运行数据为研究对象,
The effects of different dietary protein and lipid levels on juvenile greenfin horse-faced filefish (Thamnaconus septen-trionalis) was experimentally evaluated
In order to understand origin and fate of particulate organic matter,the isotopic composition(δ13C andδ15N),total or-ganic carbon content,total nitrogen content,and C/N ratios were measured for suspended particulate organic matter(PO