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对人体一氧化碳中毒和中毒程度的判断是依赖于对血中一氧化碳的分析。气相色谱是准确测定血中一氧化碳的方法。但更常用的是分光光度法。常规的分光光度法测定血中的一氧化碳时会受到碳氧血红蛋白(Hb-CO)和血红蛋白(Hb)吸收带相互重叠的影响,两者的λ_(mar)非常接近,使测得的吸收光谱实际上是两种成份各自光谱的加和,这就严重地影响到测定的准确性。为此我们试用了导数吸收光谱法测定血中一氧化碳的含量。其优点在
Judgment of carbon monoxide poisoning and poisoning in the human body depends on the analysis of carbon monoxide in the blood. Gas chromatography is an accurate method for the determination of carbon monoxide in the blood. But the more common one is spectrophotometry. Conventional spectrophotometric determination of carbon monoxide in blood is affected by the overlap of the carboxyhemoglobin (Hb-CO) and hemoglobin (Hb) absorption bands. The λ_ (mar) of the two are very close and the measured absorbance spectrum is actually On the two components of the spectrum of the sum, which severely affected the accuracy of the determination. To this end, we tried the derivative absorption spectrometry to determine the content of carbon monoxide in the blood. Its advantages are