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目的 探讨宫颈癌组织中 p16及nm2 3基因表达及其与宫颈癌生长、浸润及转移的关系。 方法 1998年 1月至 2 0 0 3年 1月广东医学院附属医院采用免疫组化 (SP)法检测 6 9例宫颈癌及 2 0例正常宫颈组织中p16和nm2 3的表达。结果 2 0例正常宫颈组织中 ,18例呈p16阳性表达 (90 0 % ) ,19例呈nm2 3阳性表达(95 0 % ) ;6 9例宫颈癌中p16和nm2 3阳性表达率分别为 5 5 1% (38/6 9)和 5 2 2 % (36 /6 9) ;p16和nm2 3阳性表达均与宫颈癌的临床分期、病理分级及淋巴结转移呈负相关 (P <0 0 1或 <0 0 5 ) ,均与宫颈癌的病理类型及年龄无关 (P >0 0 5 )。结论 p16和nm2 3的表达缺失可能在宫颈癌的发生、浸润及转移中起重要作用。p16和nm2 3检测对进一步了解宫颈癌生物学行为具有一定的临床意义
Objective To investigate the expression of p16 and nm23 genes in cervical cancer and their relationship with the growth, invasion and metastasis of cervical cancer. Methods Immunohistochemistry (SP) was used to detect the expression of p16 and nm23 in 26 cases of cervical cancer and 20 cases of normal cervical tissue from January 1998 to January 2003 in Guangdong Medical College. Results Of 20 normal cervical tissues, 18 cases showed p16 positive expression (90 0%) and 19 cases showed nm23 positive expression (95 0%). The positive rates of p16 and nm23 in 6 9 cases of cervical cancer were 5 5 1% (38/6 9) and 52 2% (36/6 9) respectively. The positive expression of p16 and nm23 were negatively correlated with the clinical stage, pathological grade and lymph node metastasis (P <0.01 or P < <0 05). All of them were not related to the pathological type and age of cervical cancer (P> 0.05). Conclusion The loss of p16 and nm23 expression may play an important role in the carcinogenesis, invasion and metastasis of cervical cancer. The detection of p16 and nm2 3 has some clinical significance for further understanding of the biological behavior of cervical cancer