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经小鼠眼球后静脉丛注射瘤细胞定量诱发实验性肺转移瘤是一个新的偿试。本文将我室近五年来实验的典型结果报道如下。材料和方法将体外原代培养的B16—黑色素瘤(引自上海药物研究所)制成单细胞悬液(台盼蓝检测其活性在95%以上),经小鼠眼球后静脉丛注射入c57BL/6小鼠(♀,18±1克,购自
It is a new test to quantitatively induce experimental lung metastases by injecting tumor cells in mouse retrobulbar venous plexus. This article will my room nearly five years of experimental results reported below. MATERIALS AND METHODS Single-cell suspension of primary cultured B16-melanoma (derived from Shanghai Institute of Materia Medica) was prepared by trypan blue assay (above 95% activity) and injected into c57BL / 6 mice (♀, 18 ± 1 g, purchased from