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目的 蛋白激酶CK2(Casein Knase2i)是一种真核细胞中普遍存在的高度保守的信使非依赖性丝/苏氨酸蛋白激酶.本研究探讨CK2催化亚基α和α′蛋白在结直肠癌组织中表达情况及临床意义.方法 采用免疫组化方法检测32例正常结直肠粘膜组织及50例结直肠癌组织中CK2α和α′蛋白的表达情况.结果 免疫组化检测结果显示,CK2α和CK2α′在结直肠癌组织中的阳性率分别为76%和64%,显著高于正常粘膜组织的阳性率125%和187%(P<005).Ⅲ-Ⅳ期结直肠癌组织中CK2α和CK2α′蛋白的阳性表达高于Ⅰ-Ⅱ期者(P>005).CK2α′蛋白的表达与癌细胞的分化程度有关(P<005),低分化组的蛋白表达高于中、高分化组.在50例结直肠癌组织中,CK2α和α′蛋白的表达呈正相关(r=0554,P<0001).结论 CK2α和α′蛋白在结直肠癌中均高表达,它们的高表达与结直肠癌的发生和发展密切相关.
Objective Casein Knase2i is a highly conserved messenger-independent silk / threonine protein kinase that is prevalent in eukaryotic cells.In this study, we explored the role of CK2 catalytic subunit α and α ’proteins in colorectal cancer tissue .Methods Immunohistochemistry was used to detect the expression of CK2α and α ’protein in 32 cases of normal colorectal mucosa and 50 cases of colorectal cancer.Results Immunohistochemical results showed that CK2α and CK2α’ The positive rates in colorectal cancer tissues were 76% and 64%, respectively, which were significantly higher than those in normal mucosa (125% vs 187%, P <005) The protein expression was higher in patients with stage Ⅰ-Ⅱ (P0.005). The expression of CK2α was related to the degree of differentiation of cancer cells (P <0.05), and the protein expression of poorly differentiated group was higher than that of moderately differentiated group The expression of CK2α and α ’protein in 50 cases of colorectal cancer was positively correlated (r = 0554, P <0001) .Conclusion CK2α and α’ proteins are highly expressed in colorectal cancer, and their high expression is correlated with colorectal cancer The occurrence and development are closely related.