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目的建立妇灵颗粒的质量标准。方法采用薄层色谱法对妇灵颗粒中木香、白术、黄柏、苦参进行定性鉴别;并应用高效液相色谱法测定芍药苷的含量。色谱柱:Kromasil(4.6mm×250mn,5μm);流动相为甲醇-0.05mol/L磷酸二氢钾溶液(40∶60);检测波长为230nm;流速:0.8ml/min;柱温:35℃。结果芍药苷在1.06～10.60μg范围内呈良好的线性关系,r=0.9999,平均回收率为98.67%,RSD=1.42%(n=6)。结论该方法简便、准确、可靠,可作为妇灵颗粒的的质量控制方法。
Objective To establish the quality standard of Fuling granule. Methods The TLC, Flos Lonicerae, Cortex Phellodendri, Radix Sophorae Flavescentis were identified qualitatively by TLC. The content of paeoniflorin was determined by high performance liquid chromatography. Column: Kromasil (4.6mm × 250mn, 5μm); mobile phase was methanol-0.05mol / L potassium dihydrogen phosphate solution (40:60); detection wavelength was 230nm; flow rate was 0.8ml / . Results Paeoniflorin showed a good linear relationship in the range of 1.06-10.60 μg with r = 0.9999. The average recovery was 98.67% with RSD = 1.42% (n = 6). Conclusion The method is simple, accurate and reliable and can be used as a quality control method for Fuling granule.