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目的:建立系统性表达CMTM2的转基因小鼠模型,研究CMTM2表达对小鼠生殖系统的影响。方法:通过显微注射pRevTRE-CMTM2表达载体建立CMTM2转基因小鼠;PCR鉴定CMTM2转基因小鼠基因型;RT-PCR、Western印迹、IHC方法检测CMTM2在转基因小鼠睾丸的表达情况;放免法检测转基因小鼠血清睾酮水平。结果:成功构建高表达CMTM2蛋白的转基因小鼠,并可以稳定传代;CMTM2转基因小鼠的血清睾酮水平较野生型小鼠提高[(46.04±3.72)nmol/L vs(42.43±3.80)nmol/L,P<0.05]。结论:成功构建CMTM2高表达的转基因小鼠,初步提示CMTM2基因可能对转基因小鼠睾酮的生成和分泌有一定影响。
Objective: To establish a transgenic mouse model that expresses CMTM2 genetically and investigate the effect of CMTM2 expression on the reproductive system in mice. Methods: The CMTM2 transgenic mice were established by microinjection of pRevTRE-CMTM2 expression vector. The genotypes of CMTM2 transgenic mice were identified by PCR. The expression of CMTM2 in testis of transgenic mice was detected by RT-PCR, Western blot and IHC. Serum testosterone levels in mice. Results: The CMTM2 protein transgenic mice were successfully constructed and stably passaged. The serum testosterone level in CMTM2 transgenic mice was significantly higher than that in wild type mice [(46.04 ± 3.72) nmol / L vs (42.43 ± 3.80) nmol / L , P <0.05]. Conclusion: The successful construction of CMTM2 high expression transgenic mice, preliminary suggests CMTM2 gene may have an impact on the generation and secretion of testosterone in transgenic mice.