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目的:探讨精神分裂症患者儿茶酚-O-甲基转移酶(COMT)第158位密码子从缬氨酸到蛋氨酸的多态性(Vall58Met)与听觉惊跳反射抑制(PPI)的关系。方法:选取符合美国精神障碍诊断与统计手册第4版(DSM-IV)的精神分裂症患者178例,正常对照190例,使用SR-HLAB惊跳反射监控系统测查听觉惊跳反射,其分析指标包括:惊跳反射的反应波幅(SR);惊跳反射的适应性(HAB);时间间隔(LI)为30 ms、60 ms、120 ms时的听觉刺激惊跳反射弱刺激抑制(PPI30%、PPI60%、PPI120%);应用聚合酶链反应和限制性片段长度多态性的方法,分析精神分裂症组与对照组COMT Vall58Met基因型与等位基因分布频率。结果:精神分裂症组的波幅(SR)低于对照组[(563±460)mVvs.(695±447)m V,P<0.05],适应性(HAB)低于对照组[(32±46)vs.(48±33),P<0.01],差异有统计学意义;精神分裂症组与对照组之间PPI差异有统计学意义(F=7.15,P<0.05),组与时间间隔的交互作用差异有统计学意义(F=5.57,P<0.05),进一步分析发现精神分裂症组的%PPI120低于对照组[(27±5)vs.(35±3),P<0.05]。2组间COMT基因型和等位基因分布有统计学意义(χ~2=8.16、11.74,均P<0.05)。COMT三种基因型对HAB%的主效应有统计学意义(F=3.07,P<0.05);分组和COMT基因型对SR,HAB%,%PPI120的交互作用无统计学意义(F=1.64、2.87、2.26,均P>0.05)。结论:COMT基因Vall58Met多态性可能与精神分裂症的适应性有关,但与精神分裂症PPI缺陷可能无关。
Objective: To investigate the relationship between valine to methionine polymorphism (Vall58Met) and auditory reflex reflex inhibition (PPI) at codon 158 of catechol-O-methyltransferase (COMT) in patients with schizophrenia. Methods: A total of 178 schizophrenic patients and 190 controls were enrolled in the DSM-IV. The SR-HLAB system was used to detect auditory reflexes. The indexes include: the response amplitude (SR) of jerky reflex; the adaptability of jerky reflex (HAB); the inhibition of weak stimuli at 30 ms, 60 ms and 120 ms of auditory stimulation (PPI30% , PPI60%, PPI120%). The genotypes and allele frequencies of COMT Vall58Met in schizophrenia group and control group were analyzed by polymerase chain reaction and restriction fragment length polymorphism. Results: The amplitude (SR) of schizophrenia group was lower than that of control group [(563 ± 460) mV vs (695 ± 447) m V, P <0.05] ) (48 ± 33), P <0.01], the difference was statistically significant. The difference of PPI between schizophrenia group and control group was statistically significant (F = 7.15, P <0.05) (F = 5.57, P <0.05). Further analysis found that% PPI120 in schizophrenia group was lower than that in control group [(27 ± 5) vs. (35 ± 3), P <0.05]. The distribution of COMT genotypes and alleles between the two groups was statistically significant (χ ~ 2 = 8.16, 11.74, both P <0.05). The main effect of COMT genotypes on HAB% was statistically significant (F = 3.07, P <0.05). There was no significant difference in the interaction between groups and COMT genotypes for SR, HAB% and% PPI120 (F = 1.64, 2.87,2.26, all P> 0.05). Conclusion: The Vall58Met polymorphism of COMT gene may be related to the adaptability of schizophrenia, but may not be related to the PPI deficiency of schizophrenia.