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目的:建立HPLC-DAD法同时测定杭白菊不同部位(茎、叶、花序)中绿原酸、3,5-O-二咖啡酰奎尼酸、蒙花苷、木犀草素-7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素、金合欢素七种活性成分的含量。方法:采用Diamonsil C18(200mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-0.2%磷酸水溶液(B),梯度洗脱,流速:1 ml·min-1,检测波长:348 nm,柱温:30℃。结果:绿原酸、3,5-O-二咖啡酰奎尼酸、蒙花苷、木犀草素-7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素、金合欢素的质量浓度分别在0.014~0.560μg(r=0.999 5)、0.031~1.260μg(r=0.999 6)、0.022~0.900μg(r=0.999 8)、0.036~1.440μg(r=0.999 6)、0.029~1.160μg(r=0.999 7)、0.005~0.220μg(r=0.999 8)和0.006~0.260μg(r=0.999 9)内与峰面积呈良好的线性关系。绿原酸、3,5-O-二咖啡酰奎尼酸、蒙花苷、木犀草素-7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素、金合欢素的平均加样回收率分别为97.91%,97.91%,97.55%,96.43%,96.44%,97.07%和97.48%,RSD分别为1.24%,1.29%,1.48%,1.83%,0.67%,1.88%和1.80%(n=6)。结论:该方法简便、准确、重复性好,可用于杭白菊药材不同部位多成分的质量控制研究。
OBJECTIVE: To establish a HPLC-DAD method for the simultaneous determination of chlorogenic acid, 3,5-O-dicaffeoylquinic acid, linarin, luteolin-7-O- β-D-glucoside, apigenin-7-O-β-D-glucoside, luteolin, acacsin the seven active ingredients. Methods: Diamonsil C18 (200mm × 4.6 mm, 5μm) was used as the mobile phase. The mobile phase consisted of acetonitrile (A) and 0.2% phosphoric acid (B), gradient elution at a flow rate of 1 ml · min- Column temperature: 30 ° C. Results: Chlorogenic acid, 3,5-O-dicaffeoylquinic acid, melphalan, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucose (R = 0.999 5), 0.031-1.260 μg (r = 0.999 6), 0.022-0.900 μg (r = 0.999 8), 0.036-1.440 (r = 0.999 6), 0.029-1.160 μg (r = 0.999 7), 0.005-0.220 μg (r = 0.999 8) and 0.006-0.260 μg (r = 0.999 9). Chlorogenic acid, 3,5-O-dicaffeoylquinic acid, glycosides, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside, The average recoveries of luteolin and acacetin were 97.91%, 97.91%, 97.55%, 96.43%, 96.44%, 97.07% and 97.48%, respectively, and the RSDs were 1.24%, 1.29%, 1.48% and 1.83% , 0.67%, 1.88% and 1.80% (n = 6), respectively. Conclusion: The method is simple, accurate, reproducible and can be used for quality control of multiple components in different parts of Chrysanthemum.