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目的 探讨18β-甘草次酸对人乳腺癌细胞(MCF-7)增殖抑制和诱导凋亡的作用,并探讨凋亡诱发与细胞内Ca2+浓度([Ca2+]i)变化的关系。方法 50-250 μmol/L浓度梯度的18β-甘草次酸处理MCF-7细胞24小时,用MTT比色法测定细胞增殖能力。100 μmol/L,150μmol/L和200 μmol/L 18β-甘草次酸处理细胞24小时,用末端脱氧核苷转移酶介导dUTP末端标记法和Annexin V流式细胞仪法检测调亡细胞。150μmol/L 18β-甘草次酸处理细胞24小时,用Fura-2荧光负载方法测定[ca2+]i的变化。结果 从100 μmol/L 18β-甘草次酸浓度起对MCF-7细胞的增殖抑制率显著升高(P<0.05和P<0.01),呈剂量依赖性,半增殖抑制浓度(IC50)为234.33 μmol/L。100 μmol/L、150 μmol/L和200 μmol/L18β-甘草次酸使细胞凋亡率显著升高(P<0.01);18β-甘草次酸处理组的[Ca2+]i也明显高于对照组(P<0.05)。结论18β-甘草次酸具有抑制MCF-7细胞增殖和诱导凋亡的作用,其诱导凋亡可能依赖于细胞内Ca2+水平上调。
Objective To investigate the inhibitory effect of 18β-glycyrrhetinic acid on the proliferation and apoptosis of human breast cancer cells (MCF-7) and to explore the relationship between apoptosis induction and intracellular Ca2 + concentration ([Ca2 +] i). Methods MCF-7 cells were treated with 18β-glycyrrhetinic acid at a concentration of 50-250 μmol / L for 24 hours. The cell proliferation was measured by MTT assay. The cells were treated with 100 μmol / L, 150 μmol / L and 200 μmol / L 18β-glycyrrhetinic acid for 24 hours. The apoptotic cells were detected by terminal deoxynucleotidyl transferase-mediated dUTP labeling and Annexin V flow cytometry. The cells were treated with 150 μmol / L 18β-glycyrrhetinic acid for 24 hours, and the change of [ca2 +] i was measured by Fura-2 fluorescence loading method. Results The proliferation inhibition rate of MCF-7 cells was significantly increased from 100 μmol / L 18β-glycyrrhetinic acid (P <0.05 and P <0.01) in a dose-dependent manner with IC50 of 234.33 μmol / L. Glycyrrhetinic acid (100 μmol / L, 150 μmol / L, 200 μmol / L) significantly increased the apoptotic rate (P <0.01) (P <0.05). Conclusion 18β-glycyrrhetinic acid can inhibit the proliferation and induce apoptosis of MCF-7 cells. The induction of apoptosis may depend on the up-regulation of intracellular Ca2 +.