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CB(calbindin-D28k),CR(calretinin)和PV(parvalbumin)是最常见的3种钙结合蛋白(calcium-binding proteins,CaBPs)。本研究首先观察了面口部给予伤害性刺激诱发大鼠延髓背角(又称三叉神经脊束核尾侧亚核)神经元表达FOS蛋白的状况;然后通过免疫荧光组织化学技术检测这些神经元内是否含有CaBPs(CB、CR和PV);最后通过免疫荧光和免疫电镜染色技术观察5-HT、GABA、甘氨酸转运体2(glycine transporter 2,GlyT2)、脑啡肽(enkephalin,ENK)或SP与CaBPs/FOS双标神经元间的联系。在光镜下可观察到:(1) FOS阳性神经元在延髓背角各层均有分布,以Ⅱ层最为密集;(2)大多数CB、CR或PV阳性神经元位于Ⅱ层,余者分布在Ⅰ层和Ⅲ层;(3) 5-HT、GABA、GlyT2,ENK及SP阳性纤维和终末主要位于延髓背角浅层(4)部分FOS阳性神经元同时呈CB、CR或PV阳性;(5) 5-HT、GABA、GlyT2或ENK阳性终末分别与FOS/CB、FOS/CR或FOS/PV双标神经元形成密切接触;(6) SP阳性终末与5-HT、GABA、GlyT2或ENK阳性终末同时与CB、CR或PV阳性神经元形成密切接触。在电镜下观察到5-HT、GABA、GlyT2或ENK阳性终末与CB、CR或PV阳性神经元主要形成对称型(抑制性)突触联系。这些结果提示在大鼠延髓背角,5-HT、GABA、甘氨酸或ENK可能通过抑制含钙结合蛋白的伤害性感受神经元来调节面口部伤害性信息的传递。
CB (calbindin-D28k), calretinin (CR) and PV (parvalbumin) are the most common three calcium-binding proteins (CaBPs). In the present study, we first observed the expression of FOS protein in nociceptive area of nociceptive neurons in the nodus of rats with facial nerve injury induced by noxious stimuli. The neurons were detected by immunofluorescence histochemistry (CB, CR and PV) were detected by immunofluorescence staining and immunoelectron microscopy. The expressions of 5-HT, GABA, glycine transporter 2 (GlyT2), enkephalin (ENK) or SP And CaBPs / FOS double labeled neurons. Observed under the light microscope: (1) FOS positive neurons in the medullary dorsal horn in each layer are distributed to the most dense layer Ⅱ; (2) most CB, CR or PV positive neurons in the second layer, the remaining (3) FOS-positive neurons of 5-HT, GABA, GlyT2, ENK and SP-positive fibers located at the superficial layer of medullary dorsal horn (4) were positive for CB, CR or PV at the same time ; (5) 5-HT, GABA, GlyT2 or ENK positive terminal were in close contact with FOS / CB, FOS / CR or FOS / PV double labeled neurons respectively; GlyT2, or ENK-positive termini are in intimate contact with CB, CR or PV-positive neurons. Observed by electron microscopy 5-HT, GABA, GlyT2 or ENK-positive terminals and CB, CR or PV-positive neurons formed mainly symmetrical (inhibitory) synaptic connections. These results suggest that 5-HT, GABA, glycine or ENK in the medullary dorsal horn of rats may regulate the transmission of nociceptive information by inhibiting the nociception of calbindin-containing neurons.