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目的 探讨锌缺乏和锌过量对培养长骨细胞周期和细胞凋亡的影响 ,为阐明锌对骨骼的生长发育影响的机制提供科学依据。方法 用小鼠胚胎长骨体外培养 ,采用流式细胞仪研究细胞周期各个时相所占比例和凋亡细胞百分比 ,进行电镜分析 ,观察细胞的超微结构。结果 缺锌可使细胞周期停滞在 G0 /G1期 ,培养 2 d,缺锌组 G0 /G1期细胞所占百分比 (82 .3% )高于对照组(76.9% ) ,G2 /M期细胞所占百分比 (3.4% )低于对照组 (8.9% ) ;培养 4d,缺锌组 G0 /G1期细胞所占百分比 (88.6% )明显高于对照组 (78.4% ) ,S期细胞所占百分比 (4.3% )明显低于对照组 (1 1 .0 % ) ;缺锌可使凋亡细胞数目增加。电镜观察 ,缺锌组可见大量的凋亡细胞 ,而高锌组则有大量的坏死细胞。结论 锌缺乏可改变培养长骨的细胞周期 ,诱导细胞凋亡 ,但锌过量对细胞周期和细胞凋亡没有影响。
Objective To investigate the effects of zinc deficiency and zinc overdose on cell cycle and apoptosis in cultured long bone cells and to provide a scientific basis for elucidating the mechanism of zinc on bone growth and development. Methods The long bones of mouse embryos were cultured in vitro. The proportion of apoptotic cells and the percentage of apoptotic cells in each phase of the cell cycle were studied by flow cytometry. The ultrastructure of the cells was observed by electron microscopy. Results The cell cycle arrest in G0 / G1 phase was induced by zinc deficiency. After cultured for 2 days, the percentage of G0 / G1 phase cells in zinc deficiency group (82.3%) was higher than that in control group (76.9%), G2 / M phase (3.4%) was lower than that of the control group (8.9%). After cultured for 4 days, the percentage of G0 / G1 phase cells in the zinc deficiency group was significantly higher than that in the control group (88.6% vs 78.4% 4.3%) was significantly lower than the control group (11.0%); zinc deficiency can make the number of apoptotic cells increased. Electron microscopy showed a large number of apoptotic cells in the zinc deficiency group, while a large number of necrotic cells in the high zinc group. Conclusion Zinc deficiency can change the cell cycle of cultured long bones and induce apoptosis. However, zinc deficiency has no effect on cell cycle and apoptosis.