本研究从抗冷玉米自交系W9816中克隆了Zm GDH基因。分析发现该基因与其他植物中的谷氨酸脱氢酶基因(GDH)基因具有较高的同源性,其中与高粱同源性达到95.38%。对抗冷玉米自交系W9816进行4℃冷处理,分别在处理后0、6 h、12 h、24 h和48 h取材,采用q RT-PCR方法测定不同冷处理时间下Zm GDH在根、茎、叶中的表达情况,结果发现在不同组织中Zm GDH基因对不同时长的冷诱导响应存在显著的差异。另外通过农杆菌介导法对玉米骨干自交系Y423愈伤组织进行了Zm GDH基因的转化,PCR检测鉴定阳性转化植株,最终统计阳性转化率为4.3%。对T0代阳性植株进行q RT-PCR表达量分析,证明了阳性转化植株中Zm GDH基因的高表达。本研究所获得的Zm GDH转基因材料可用于Zm GDH基因的生物功能分析及抗冷转基因玉米新种质创制。 In this study, Zm GDH gene was cloned from the resistant maize inbred line W9816. The results showed that this gene shared high homology with glutamate dehydrogenase gene (GDH) in other plants, with homology of 95.38% to sorghum. Cold treatment of cold maize inbred line W9816 was carried out at 4 ℃ for 0, 6 h, 12 h, 24 h and 48 h, respectively. The q RT-PCR method was used to determine the effect of Zm GDH on root, stem and leaf The results showed that Zm GDH gene in different tissues showed significant differences in cold-induced response to different durations. In addition, Zm GDH gene was transformed into Y423 maize callus by Agrobacterium tumefaciens-mediated method. The positive transgenic plants were identified by PCR. The positive conversion rate was 4.3%. Q RT-PCR analysis of T0 generation positive plants showed that Zm GDH gene was highly expressed in the positive transgenic plants. The Zm GDH transgenic material obtained in this study can be used for biological function analysis of Zm GDH gene and creation of new germplasm of cold-resistant transgenic maize.