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目的:结合斑蝥主要成分斑蝥素的含量测定与指纹图谱,比较不同产地药材化学成分的差异性,为科学评价不同产地斑蝥药材及其超微粉体的质量提供依据。方法:建立斑蝥超微粉体指纹图谱检测方法。采用高效液相色谱(HPLC)法对各产地斑蝥超微粉体进行分析,采用中药色谱指纹图谱相似度评价系统软件比较指纹图谱的相似度,采用SPSS 13.0统计软件对10批样品进行聚类分析,并测定斑蝥素含量。结果:不同产地斑蝥超微粉体指纹图谱有较大相似性,但斑蝥素的含量有一定的差异。聚类分析结果表明,不同产地斑蝥药材存在一定差异,但差别不大。结论:所建立的HPLC指纹图谱具有较好的精密度、重复性和稳定性,结合斑蝥素的含量,可为斑蝥药材及其超微粉体质量评价提供依据。
OBJECTIVE: To compare the content of cantharidin and fingerprinting of the main constituents of Cantharidin, and to compare the differences in the chemical components of medicinal materials from different areas so as to provide basis for the scientific evaluation of the quality of cantharidin and its ultrafine powders in different areas. Methods: To establish the method of detecting the fingerprint of Cantharidin ultrafine powder. High-performance liquid chromatography (HPLC) was used to analyze the cantharidin superfine powder from different producing areas. The similarity of the fingerprints was compared using the similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine. The data of 10 batches were analyzed by SPSS 13.0 software , And determine cantharidin content. Results: The fingerprints of the cantharidin superfine powder in different areas have similarities, but the content of cantharidin is different. The result of cluster analysis shows that there are some differences among the medicinal materials of mackerel in different producing areas, but the difference is not obvious. Conclusion: The established HPLC fingerprint has good precision, repeatability and stability. Combining with the content of cantharidin, HPLC fingerprinting can provide the basis for the quality evaluation of mackerel and its ultrafine powder.