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目的:探讨内淋巴囊对外源性胸腺依赖性(TD)抗原的吞噬和清除作用。方法:用S-D大鼠30只,以TD抗原钥孔(?)血蓝蛋白(KLH)全身免疫后2周,再次在内耳接种相同抗原,分别于此后1 h、3 h、1 d、3 d、7 d和14 d,取颞骨作组织学处理、冰冻切片,用抗KLH单克隆抗体,运用免疫组织化学技术,观察内耳免疫应答过程中内淋巴囊腔内KLH的动态变化。结果:内耳抗原接种后3 h,内淋巴囊出现KLH;第1～7 d在内淋巴囊的抗原被捕捉吞噬和递呈;第14天内淋巴囊仅发现微弱的抗原染色。结论:在内耳的二次应答中,内淋巴囊能够吞噬、处理和清除外源性抗原说明其具有免疫防御和免疫应答功能。
Objective: To investigate the phagocytosis and clearance of exogenous thymus-dependent (TD) antigen by endolymphatic sac. Methods: Thirty Sprague-Dawley rats were immunized twice a week with systemic immunization with keyhole limpet hemocyanin (KLH). The same antigen was inoculated into the inner ear again, and then were exposed to the same antigen at 1 h, 3 h, 1 d, 3 d , 7 d and 14 d. The temporal bone was taken for histological treatment. Frozen sections were obtained. The anti-KLH monoclonal antibody and immunohistochemical technique were used to observe the dynamic changes of KLH in the endolymphatic sac cavity during the inner ear immune response. RESULTS: KLH was found in the endolymphatic sac 3 h after inoculation of the inner ear antigen. The antigen in the endolymphatic sac was captured and phagocytosed from day 1 to day 7, and only weak antigen staining was found on the 14th day. CONCLUSION: In the secondary response of the inner ear, the endolymphatic sac can swallow, process and remove exogenous antigens indicating that it has immune defense and immune response functions.