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本文研究的主要目的是要取得神经移植成功的组织学和组织化学的证据。作者对16只狗施行了各种类型实验性环杓后肌神经移植法——喉返神经自身吻合6例;颈神经袢移植5例;膈神经移植5例。于3～7个月记录声带功能后,处死实验狗,切除两侧环杓后肌做组织化学研究。将肌肉即刻放于异戊烷中冰冻,在液氮中冷却至冰点,把每块环杓后肌切成4片,两侧相对应的肌片一起制成10μm厚的冰冻切片,孵育切片检查肌动球蛋白三磷酸腺苷酶(actomyosin ATPase)、烟酰胺腺嘌呤二核苷酸四唑还原酶(NADH-TR)和甲基甘油磷酸脱氢酶(α-GPDH),用绘图法计算出不同类型肌纤维的百分数。在每块环杓后肌中挑选代表区,每区包含约150根肌纤维,用光度计测
The main purpose of this study is to obtain evidence of histological and histochemical success of neural transplantation. The authors performed various types of experimental posterior circumflex ciliary nerve transplantation in 16 dogs: 6 cases of recurrent laryngeal nerve autograft; 5 cases of cervical nerve graft; 5 cases of phrenic nerve graft. After recording the vocal cord function for 3 to 7 months, the experimental dogs were sacrificed and the posterior circumferior bones were removed for histochemical studies. The muscle was immediately frozen in isopentane, cooled to freezing point in liquid nitrogen, and each posterior member of the crab was cut into 4 pieces, and the corresponding muscle pieces on both sides were made into 10 μm-thick frozen sections, and the sections were examined by incubation Actomyosin ATPase, nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR), and methylglycerophosphate dehydrogenase (α-GPDH), were used to calculate the different types of muscle fibers Of the percentage. A representative area was selected from each posterior ciliate and each area contained approximately 150 myofibers, measured with a photometer