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目的 研究卵巢切除术诱导的绝经后骨质疏松症大鼠模型股骨颈质量的力学表达与附着肌群肌强度的基因表达的变化 ,探讨股骨颈骨质疏松性骨折风险升高的肌肉 骨骼机制。 方法 2 0只 8月龄Wistar雌性大鼠随机分为对照组 10只 ,双侧卵巢假切除 ;模型组 10只为双侧卵巢切除。手术后第 180天 ,分别测定股骨颈质量的结构强度、结构硬度和结构韧性与附着股骨颈的外展肌群肌强度的重链肌球蛋白基因的表达。 结果 模型组的结构强度 ( 168 0 9± 2 5 2 0 )N、结构硬度( 682 3 0± 13 1 2 7)N/mm和结构韧性 ( 0 0 4± 0 0 7)J与对照组比较都显著下降 (P <0 0 5) ;附着的外展肌群肌强度的基因表达 ,对照组重链肌球蛋白ImRNA表达量高 ,模型组表达量低。 结论 股骨颈质量的低结构强度、低结构硬度与低结构韧性的力学表达导致股骨颈骨质疏松性骨折风险升高 ;附着肌群肌强度的重链肌球蛋白ImRNA低表达对于造成骨质量力学表达变化的骨重建高转换的调控机制具有重要的作用。提示降低股骨颈骨质疏松性骨折风险的临床干预策略必须考虑“筋、骨齐治”。
OBJECTIVE: To study the changes of mechanical expression of femoral neck mass and the gene expression of myofascial muscle attached to ovariectomy-induced osteoporosis rat model and to explore the musculoskeletal mechanism of increased femoral neck osteoporotic fracture risk. Methods Twenty female Wistar rats of 8 months old were randomly divided into control group (n = 10) and bilateral ovary resection. The model group (n = 10) was bilateral ovariectomy. On the 180th day after surgery, the expression of the heavy chain myosin gene of the femoral neck, the structural strength, the structural rigidity, the structural toughness, and the extensor muscle strength of the femoral neck were measured. Results Compared with the control group, the structural strength of the model group (168 0 9 ± 25 20) N, the structural rigidity (682 30 ± 13 1 27) N / mm and the structural toughness (0 0 4 ± 0 0 7) J (P <0.05). The gene expression of muscular strength of the attached abductor muscles was high. The mRNA expression of heavy chain myosin ImRNA in the control group was high and the expression level of the model group was low. CONCLUSIONS: The low structural strength of the femoral neck, the low structural rigidity and low structural toughness of the mechanical expression lead to an increased risk of femoral neck osteoporotic fracture. The low muscle myoglobin mRNA expression of myosin ImRNA may contribute to bone mass mechanics Expression and regulation of bone remodeling mechanism of high conversion has an important role. Tips to reduce femoral neck osteoporotic fracture clinical intervention strategy must consider “tendons, bone Qi governance.”