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矮秆基因的发掘、研究和利用是水稻株型改良育种和植物生长发育分子生物学研究的重要基础.利用新桂矮双矮与02428杂交产生的F2群体对sd-g进行了精细定位.sd-g基因首先被定位在水稻第5染色体上微卫星标记RM440和RM163之间,遗传距离分别是0.5和2.5cM.为了进一步精细定位sd-g基因,利用已经公布的水稻基因组序列,在sd-g基因附近区域寻找微卫星序列并发展新的标记,在RM440和RM163之间发展了9个微卫星标记.sd-g基因被进一步定位在SSR5-1和SSR5-51之间,SSR5-1与sd-g之间相距0.1cM,SSR5-51与sd-g之间相距0.3cM,而SSR418与sd-g表现为共分离.以此为基础,构建覆盖sd-g基因区域的BAC重叠群,sd-g基因被定位在AC105319约85kb的区段上,这为sd-g基因的图位克隆奠定了基础.
The discovery, research and utilization of dwarfing genes are important bases for the molecular breeding of plant type and plant growth and development in sd-g. The-g gene was first located on microsatellite markers RM440 and RM163 on chromosome 5 of rice with genetic distances of 0.5 and 2.5 cM, respectively. In order to further finely locate sd-g gene, the published sequence of rice genome was used to amplify sd- In the vicinity of g gene, we searched for microsatellite sequences and developed new markers, and developed 9 microsatellite markers between RM440 and RM163. The sd-g gene was further mapped between SSR5-1 and SSR5-51, and SSR5-1 sd-g and SSR5-51 were 0.3cM and sd-g, respectively, whereas SSR418 and sd-g showed co-segregation.According to this, BAC contigs covering the sd-g gene region were constructed, The sd-g gene was located on the segment of about 85kb of AC105319, which laid the foundation for the map-based cloning of sd-g gene.