作为2015版《中国药典》项目——豆豉姜的质量标准增订工作的一部分,本研究对豆豉姜进行了化学成分研究。利用硅胶、Sephadex LH-20、ODS等色谱技术分离得到了7个异喹啉生物碱,并通过MS,1D和2D NMR波谱数据分析及与文献相比对等方法,分别鉴定为(+)-去甲波尔定(1)、(+)-波尔定(2)、(+)-瑞枯灵(3)、(+)-六驳碱(4)、(+)-异波尔定(5)、(+)-N-甲基六驳碱(6)、小檗碱(7),其中化合物7为首次从本属中分离得到。利用BV-2和RAW264.7细胞炎症模型评价其体外抗炎作用,化合物1,2,5和7显示中度至微弱的活性。 As part of the revision of the quality standard of the Chinese Pharmacopoeia 2015 edition, the research on the chemical constituents of Gleditsia chensinensis was carried out. Seven isoquinoline alkaloids were isolated by chromatography on silica gel, Sephadex LH-20 and ODS. The relative isoquinolines were identified as (+) - (+) - irbesartan (3), (+) - hexamine (4), (+) - isoporodine (5), (+) - N-methyl hexamine (6) and berberine (7), of which compound 7 was isolated from this genus for the first time. The in vitro anti-inflammatory effects were evaluated using the BV-2 and RAW264.7 cell inflammation models and compounds 1, 2, 5 and 7 showed moderate to weak activity.